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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM17 antibody (ab57484)
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Western blot - ADAM17 antibody (ab57484)
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Immunocytochemistry/ Immunofluorescence - ADAM17 antibody (ab57484)
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Product Name
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ADAM17 antibody
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See all ADAM17 antibodies (12)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to ADAM17
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Immunogen
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Recombinant fragment: RADPDPMKNT CKLLVVADHR FYRYMGRGEE STTTNYLIEL IDRVDDIYRN TSWDNAGFKG YGIQIEQIRI LKSPQEVKPG EKHYNMAKSY PNEEKDAWDV , corresponding to amino acids 215-315 of Human ADAM17
BLAST 'RADPDPMKNT CKLLVVADHR FYRYMGRGEE STTTNYLIEL IDRVDDIYRN TSWDNAGFKG YGIQIEQIRI LKSPQEVKPG EKHYNMAKSY PNEEKDAWDV ' with  or 
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Reacts with
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at a concentration of 3 µg/ml.
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; single pass type I membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Signal transduction >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Stem Cells >> Signaling Pathways >> Notch >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Relevance
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ADAM17, also known as TNF alpha Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other post translational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAM17 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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ADAM17 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM17 antibody (ab57484)
ADAM17 antibody (ab57484) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
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Western blot - ADAM17 antibody (ab57484)
Western blot against tagged recombinant protein immunogen using ab57484 ADAM17 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
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Immunocytochemistry/ Immunofluorescence - ADAM17 antibody (ab57484)
ab57484 was used to detect mouse ADAM17 overexpressed in Hela cells. The transfected cells were incubated with ab57484 (10 µg/ml ) for 1 hour at 22°C. For further details please refer to the Abreview. Image taken from an Abreview published in February 2008
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References for ADAM17 antibody (ab57484)
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This product has been used in:
Gutwein P et al. CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein. Am J Pathol 174:2061-72 (2009). ICC/IF; Human. PubMed: 19435795
If you publish research using ab57484 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ADAM17
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ADAM17 antibody - more information
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