Anti-ADAM17 antibody (ab57484)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM17 antibody (ab57484)

ADAM17 antibody (ab57484) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.

Western blot - ADAM17 antibody (ab57484)

Western blot against tagged recombinant protein immunogen using ab57484 ADAM17 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

Immunocytochemistry/ Immunofluorescence - ADAM17 antibody (ab57484)

ab57484 was used to detect mouse ADAM17 overexpressed in Hela cells. The transfected cells were incubated with ab57484 (10 µg/ml ) for 1 hour at  22°C. For further details please refer to the Abreview.

Image taken from an Abreview published in February 2008

Flow Cytometry - ADAM17 antibody (ab57484)

Overlay histogram showing HeLa cells stained with ab57484 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57484, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.