Overview

  • Product nameAnti-ADAM17 antibodySee all ADAM17 primary antibodies ...
  • Description
    Rabbit polyclonal to ADAM17
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human ADAM17.

    (Peptide available as ab94552.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lyates: HeLa; Jurkat; MDA MB 231, as well as the following tissue lysates; Human liver; Human heart; Human placenta; Human skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab77820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 124 kDa (predicted molecular weight: 93 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
    • Tissue specificityUbiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
    • Sequence similaritiesContains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
    • DomainMust be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
      Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
    • Cellular localizationMembrane.
    • Target information above from: UniProt accession P78536 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
      • A disintegrin and metalloproteinase domain 17 antibody
      • ADA17_HUMAN antibody
      • ADAM 17 antibody
      • ADAM metallopeptidase domain 17 antibody
      • ADAM17 antibody
      • ADAM17 protein antibody
      • CD 156b antibody
      • CD156b antibody
      • CD156b antigen antibody
      • CSVP antibody
      • Disintegrin and metalloproteinase domain-containing protein 17 antibody
      • MGC71942 antibody
      • Snake venom like protease antibody
      • Snake venom-like protease antibody
      • TACE antibody
      • TNF alpha convertase antibody
      • TNF alpha converting enzyme antibody
      • TNF-alpha convertase antibody
      • TNF-alpha-converting enzyme antibody
      • Tumor Necrosis Factor Alpha Converting Enzyme antibody
      see all

    Anti-ADAM17 antibody images

    • All lanes : Anti-ADAM17 antibody (ab77820) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
      Lane 5 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
      Lane 6 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
      Lane 7 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 93 kDa
      Observed band size : 124 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 18 kDa,30 kDa,69 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 2 minutes

    References for Anti-ADAM17 antibody (ab77820)

    ab77820 has not yet been referenced specifically in any publications.

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