Anti-ADAM17 antibody (ab77820)

Overview

• Product nameAnti-ADAM17 antibodySee all ADAM17 primary antibodies ...
• Description
  Rabbit polyclonal to ADAM17
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human ADAM17.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab94552.)

• Positive controlThis antibody gave a positive signal in the following whole cell lyates: HeLa; Jurkat; MDA MB 231, as well as the following tissue lysates; Human liver; Human heart; Human placenta; Human skeletal muscle.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Types of disease
  • Cancer

  • Stem Cells
  • Signaling Pathways
  • Notch
  • Secreted

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • ADAM protein family

  • Cancer
  • Signal transduction
  • Other

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Signal Transduction
  • Adapters
  • Transmembrane

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Metalloprotease
  • ADAMs

Applications

Target

• FunctionCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
• Tissue specificityUbiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
• Sequence similaritiesContains 1 disintegrin domain.
  Contains 1 peptidase M12B domain.
• DomainMust be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
  The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
• Post-translational
  modificationsThe precursor is cleaved by a furin endopeptidase.
  Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
• Cellular localizationMembrane.

Target information above from: UniProt accession P78536 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6868 Human
  • Omim: 603639 Human
  • SwissProt: P78536 Human
  • Unigene: 404914 Human

• Alternative names
  A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibodyA disintegrin and metalloproteinase domain 17 antibodyADA17_HUMAN antibody

  ADAM 17 antibodyADAM metallopeptidase domain 17 antibodyADAM17 antibodyADAM17 protein antibodyCD 156b antibodyCD156b antibodyCD156b antigen antibodyCSVP antibodyDisintegrin and metalloproteinase domain-containing protein 17 antibodyMGC71942 antibodySnake venom like protease antibodySnake venom-like protease antibodyTACE antibodyTNF alpha convertase antibodyTNF alpha converting enzyme antibodyTNF-alpha convertase antibodyTNF-alpha-converting enzyme antibodyTumor Necrosis Factor Alpha Converting Enzyme antibody

  see all

Anti-ADAM17 antibody images

• 

  Western blot - ADAM17 antibody (ab77820)
  All lanes : Anti-ADAM17 antibody (ab77820) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
  Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
  Lane 5 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
  Lane 6 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
  Lane 7 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 93 kDa
  Observed band size : 124 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 18 kDa,30 kDa,69 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 2 minutes