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ADAM17 peptide (Cytoplasmic domain) (ab41214)

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Overview

  • Product nameADAM17 peptide (Cytoplasmic domain)See all ADAM17 proteins and peptides ...
  • Protein descriptionSynthetic peptide ADAM17. (Note: the amino acid sequence is proprietary) This peptide was used as an immunogen for ab39162 - ADAM17 antibody - Cytoplasmic domain.

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab41214 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA
    WB
  • Application notesELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab39162. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        A disintegrin and metalloproteinase domain 17A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme)ADA17_HUMAN
        ADAM 17ADAM metallopeptidase domain 17ADAM17ADAM17 proteinCD 156bCD156bCD156b antigenCSVPDisintegrin and metalloproteinase domain-containing protein 17MGC71942Snake venom like proteaseSnake venom-like proteaseTACETNF alpha convertaseTNF alpha converting enzymeTNF-alpha convertaseTNF-alpha-converting enzymeTumor Necrosis Factor Alpha Converting Enzyme
      see all
  • FunctionCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • Tissue specificityUbiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • DomainMust be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P78536 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for ADAM17 peptide (Cytoplasmic domain) (ab41214)

    ab41214 has not yet been referenced specifically in any publications.

    Publishing research using ab41214 ? Please let us know so that we can cite the reference in this datasheet

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    Our user would like to ADAM17 peptide(ab41213, 41214 and 41215). Before order, she wants know these sequnce. Could you tell me sequnce of ab41213, 41214 and 41215? Please check this for our user. Thank you for your consideration all the time. ...

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    Thank you for your inquiry. Below is the approximate sequence information: ab41213- propeptide: immunogenic peptide is in the first half of the propeptide domain of human ADAM-17 (propeptide form is 18 – 214aa) ab41214-cytoplasmic domain- within t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"