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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
Anti-ADAM20 antibody - Catalytic domain
See all ADAM20 products (4) ...
Description
Rabbit polyclonal to ADAM20 - Catalytic domain
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the catalytic domain of human ADAM20.
(Peptide available as ab41221.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Western blot - ADAM20 antibody - Catalytic domain (ab28295)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: A recommended starting concentration for Western Blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 82 kDa. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. This product recognizes bands of 95 kDa, 84 kDa (major band), and breakdown products at 50 kDa, 34 kDa in reduced Western Blots of cell lysates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in sperm maturation and/or fertilization.
Tissue specificity
Testis specific.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
Domain
A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
Cellular localization
Membrane.
Target information above from: UniProt accessionO43506
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 20 preproprotein antibody
- ADA20_HUMAN antibody
- ADAM 20 antibody
see all
Database links
- Entrez Gene: 8748 Human
- Omim: 603712 Human
- SwissProt: O43506 Human
- SwissProt: Q6GTZ1 Human
- Unigene: 177984 Human
Anti-ADAM20 antibody - Catalytic domain images:
Western blot - ADAM20 antibody - Catalytic domain (ab28295)
All lanes : Anti-ADAM20 antibody - Catalytic domain (ab28295) at 1/1000 dilution
Lane 1 : Mouse Tetis Lysate
Lane 2 : Mouse Epididymis Lysate
Lysates/proteins at 20 µl per lane.
Predicted band size : 82 kDa
Protocols
References for Anti-ADAM20 antibody - Catalytic domain (ab28295)
ab28295 has not yet been referenced specifically in any publications.
Publishing research using ab28295? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"