Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
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All lanes : Anti-ADAM28 antibody - Aminoterminal end (ab28292) at 1 µg/ml
Lane 1 :
Lane 2 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 87 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 12 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
The band observed at 66 kDa could potentially be a cleaved form of ADAM28 due to the presence of an 18 amino acid signal peptide and a 180 amino acid propeptide.
1
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