Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAM28
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ADAM28 (internal sequence). (NP_055080).
    Database link: Q9UKQ2

  • Positive control
    • Human breast cancer and colon cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab198902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.

Target

  • Relevance
    ADAM28, also known as eMDCII, MDC-Ls and TECADAM, was first described in lymphocytes, also in Thymic Endothelial Cells (TECADAM), as well as in the epididymus. Later works describe two forms of ADAM28: ADAM28-S and ADAM28-L (short and long forms). The short form is a soluble, alternatively spliced form lacking the transmembrane and cytoplasmic domains. Message levels of the short form were found to be much lower than the long form in the tissues studied, but these may be tissue-specific differences. In humans, ADAM28 is reported in lymphocytes by some authors and to be absent from mature lymphocytes by others. The thyroid, lung, trachea, esophagus, and stomach have been shown to express human ADAM28 message. Knockout mice lacking B cells and T cells express ADAM28 message in the thyroid, lung, trachea, esophagus, and stomach. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the functions of ADAM28 are still poorly understood. ADAM28 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM28 (ADAM28 Long) sequence codes for a 775 amino acid protein with a predicted mass is 87.2 kDa, and a pI of 6.50. The soluble ADAM28 (ADAM-28 Short) is a 540 amino acid sequence, with a predicted mass of 61.3 kDa, and a pI of 6.58. Other forms of ADAM28 have been reported with slightly different carboxyterminal regions. ADAM28 may play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. It may be involved in sperm maturation. There are 2 isoforms of ADAM28: one short secreted and one long membrane bound.
  • Cellular localization
    Cell Membrane and Secreted
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 28 antibody
    • ADAM 28 antibody
    • ADAM metallopeptidase domain 28 antibody
    • ADAM23 antibody
    • Disintegrin and metalloproteinase domain containing protein 28 antibody
    • eMDC II antibody
    • eMDCII antibody
    • epididymal Metalloproteinase like disintegrin like and cysteine rich protein II antibody
    • MDC L antibody
    • MDCL antibody
    • Metalloproteinase like disintegrin like and cysteine rich protein L antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ADAM28 with ab198902 at 1/30 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling ADAM28 with ab198902 at 1/30 dilution.

References

ab198902 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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