ADAM30 antibody - Metalloproteinase domain (ab40136)

Product Name 

ADAM30 antibody - Metalloproteinase domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAM30 - Metalloproteinase domain

Immunogen 

Synthetic peptide based on the metalloproteinase domain of human ADAM30.

(Peptide available as ab41226.)

Reacts with 
(species key)

Hu

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all ADAM30 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, 1/5000 for chemiluminescent substrates. Predicted molecular weight: 89 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; single pass type I membrane protein

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family

Relevance 

ADAM30 was first cloned along with ADAM29 from human testis cDNA, in a search for novel ADAMs. The ADAM29 and ADAM30 sequences are dissimilar, and share 37% identity on the amino acid level. Two different ADAM30 sequences were identified in the initial paper, 790 amino acids and 781 amino acids. The 9 amino acid deletion in the shorter form is in the cytoplasmic domain, and may affect interaction with cytoplasmic elements. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs), the function of ADAM23 is still poorly understood. ADAM30 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and is not thought to be proteolytically active. ADAM29 does not have this conserved zinc binding motif, and it is tempting to speculate that the two proteins form a heterodimer, such as ADAM1 and ADAM2 do (although there is no direct evidence to date for such an interaction). Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM30 sequence codes for a 790 amino acid protein, containing a Type I transmembrane domain, with a predicted mass of 88.94 kDa and a pI of 8.16. The shorter form is 781 amino acids, with predicted mass of 88 kDa and 8.50 pI.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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