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Anti-ADAM30 antibody - Metalloproteinase domain (ab40136)

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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab40136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, 1/5000 for chemiluminescent substrates. Predicted molecular weight: 89 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceADAM30 was first cloned along with ADAM29 from human testis cDNA, in a search for novel ADAMs. The ADAM29 and ADAM30 sequences are dissimilar, and share 37% identity on the amino acid level. Two different ADAM30 sequences were identified in the initial paper, 790 amino acids and 781 amino acids. The 9 amino acid deletion in the shorter form is in the cytoplasmic domain, and may affect interaction with cytoplasmic elements. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs), the function of ADAM23 is still poorly understood. ADAM30 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and is not thought to be proteolytically active. ADAM29 does not have this conserved zinc binding motif, and it is tempting to speculate that the two proteins form a heterodimer, such as ADAM1 and ADAM2 do (although there is no direct evidence to date for such an interaction). Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM30 sequence codes for a 790 amino acid protein, containing a Type I transmembrane domain, with a predicted mass of 88.94 kDa and a pI of 8.16. The shorter form is 781 amino acids, with predicted mass of 88 kDa and 8.50 pI.
    • Cellular localizationMembrane; single pass type I membrane protein
    • Database links
      • Alternative names
          A disintegrin and metallopeptidase domain 30 antibodyADAM metallopeptidase domain 30 antibodyDisintegrin and metalloproteinase domain-containing protein 30 antibody
          EC 3.4.24.- antibodyMGC132801 antibodyMGC132802 antibodyOTTHUMP00000014034 antibodySvph4 antibody
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      References for Anti-ADAM30 antibody - Metalloproteinase domain (ab40136)

      ab40136 has not yet been referenced specifically in any publications.

      Publishing research using ab40136 ? Please let us know so that we can cite the reference in this datasheet

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      What is the peptide sequences recognised by : ab28468, ab28290, ab28288,ab38944, ab39879, ab40136, ab28295, ab40132, ab28293 ?

      Dear Cecil In response to your query, I'm afraid I can't provide you with more specific details on the peptide sequence because these amino acid sequences are proprietary. The only information I can provide you with is the following details: # ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"