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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ADAM33 antibody - Cytoplasmic domain(ab39193)
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Product Name
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ADAM33 antibody - Cytoplasmic domain
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See all ADAM33 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADAM33 - Cytoplasmic domain
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Immunogen
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Synthetic peptide from Human ADAM33 based on the cytoplasmic domain.
(Peptide available as ab41229.)
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Reacts with
(species key)
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Hu
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Specificity
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We have a range of domain specific antibodies for this target. For a full list please see all ADAM33 antibodies
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Tested applications
(see key)
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ICC/IF, IHC-R, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-R: Use at an assay dependent dilution.
IHC-P: Use at 0.5ug/ml (see ref Susan C. Foley MD et al)
WB: 1/1000 - 1/5000. Detects a band of approximately 99 kDa (minor form), due to glycosylation and cysteine rich regions, and a band at 58 kDa (major band), under reducing conditions. (Predicted molecular weight: 88 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Relevance
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ADAM33, also known as A disintegrin and metalloproteinase 33, is a member of the metalloproteinase family containing disintegrin-like domains (ADAMs). ADAM33 appears to be widely expressed and found in most organs. ADAM33 may act as a cell-attachment molecule, by binding integrins through the cysteine-rich domain. Recent work suggests that mutations in ADAM33 may be linked to asthma and that many of the mutations generate new cysteine residues, which may disturb the function of ADAM33.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody has been peptide-affinity purified.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAM33 antibody - Cytoplasmic domain is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADAM33 antibody - Cytoplasmic domain images, references, products related to ab39193 and other tools.
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ADAM33 antibody - Cytoplasmic domain images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ADAM33 antibody - Cytoplasmic domain(ab39193)
Ab39193 staining human normal myometrium. Staining is localized to the membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for ADAM33 antibody - Cytoplasmic domain (ab39193)
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This product has been used in: (two most recent references)
Haitchi HM et al. ADAM33 expression in asthmatic airways and human embryonic lungs. Am J Respir Crit Care Med 171:958-65 (2005). PubMed: 15709049
Susan C. Foley MD et al Increased expression of ADAM33 and ADAM8
If you publish research using ab39193 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ADAM33
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