|
|
|
|
Western blot - ADAMTS1 antibody - Aminoterminal end (ab28284)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence-ADAMTS1 antibody - Aminoterminal end(ab28284)
(enlarge)
|
|
|
|
|
Product Name
|
ADAMTS1 antibody - Aminoterminal end
|
| |
See all ADAMTS1 antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ADAMTS1 - Aminoterminal end
|
|
Immunogen
|
Synthetic peptide based on the aminoterminal end of furin cleaved human ADAMTS-1.
(Peptide available as ab41230.)
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
This antibody recognizes the zymogen and active forms of ADAMTS 1.
We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS1 antibodies
|
|
Epitope
|
This antibody recognizes the aminoterminal end of
furin cleaved ADAMTS-1, after the second furin cleavage site.
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 - 1/5000. Detects bands of approximately 110-120 (due to glycoslylation and cysteine rich regions of zymogen form) and a 84-98 kDa band, (active form, major band). (Predicted molecular weight: 106 kDa). Note: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Secreted. Associated with the extracellular matrix.
|
|
Research areas
|
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
|
|
Relevance
|
ADAMTS1 is a metalloproteinase of the ADAM (A Disintegrin And Metalloproteinase) family containing disintegrin-like domains. ADAMTS1, also known as METH1, was first described as a protein elevated in invasive mouse tumors. Initial findings indicated a role for ADAMTS1 in tumor progression, since the protein was preferentially expressed in more invasive tumor cell lines. ADAMTS1 is necessary for normal growth, fertility, and organ morphology and function.
|
|
|
ADAMTS1 antibody - Aminoterminal end (ab28284) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
This antibody has been peptide-affinity purified.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS1 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ADAMTS1 antibody - Aminoterminal end images, references, products related to ab28284 and other tools.
|
| |
ADAMTS1 antibody - Aminoterminal end images:
|
 Western blot - ADAMTS1 antibody - Aminoterminal end (ab28284)
ADAMTS1 antibody - Aminoterminal end (ab28284) at 1/2500 dilution + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 106 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
|
| |
Immunocytochemistry/ Immunofluorescence-ADAMTS1 antibody - Aminoterminal end(ab28284)
ICC/IF image of ab28284 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28284, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
| |
|
Search PubMed (MEDLINE) for references to ADAMTS1
|
| |
ADAMTS1 antibody - Aminoterminal end - more information
|
Customer reviews (feedback) regarding ADAMTS1 antibody - Aminoterminal end |
Customer FAQs regarding ADAMTS1 antibody - Aminoterminal end |
Protocols for ADAMTS1 antibody - Aminoterminal end |
Price and availability of products related to ADAMTS1 antibody - Aminoterminal end |
| |
ADAMTS1 antibody - Aminoterminal end - related products:
|
|
|
| |
ADAMTS1 antibody - Aminoterminal end - other tools:
|
Contact Abcam with a Technical Enquiry about ADAMTS1 antibody - Aminoterminal end |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
