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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-ADAMTS1 antibody - Aminoterminal end
See all ADAMTS1 products (5) ...
Description
Rabbit polyclonal to ADAMTS1 - Aminoterminal end
Specificity
This antibody recognizes the zymogen and active forms of ADAMTS 1.
Tested applications
IHC-P, Neutralising, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human, Rhesus monkey
Immunogen
Synthetic peptide based on the aminoterminal end of furin cleaved human ADAMTS-1.
(Peptide available as ab41230.)
Epitope
This antibody recognizes the aminoterminal end of furin cleaved ADAMTS-1, after the second furin cleavage site.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Neut: Use at an assay dependent dilution. (PubMed: 20840749)
WB: 1/1000 - 1/5000.Detects a band of approximately 110-120 , 84-98 kDa (predicted molecular weight: 106 kDa).(Detects bands of approximately 110-120 (due to glycoslylation and cysteine rich regions of zymogen form) and a 84-98 kDa band, (active form, major band). (Predicted molecular weight: 106 kDa). Note: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. )
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ9UHI8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 1 antibody
- A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1 antibody
see all
Database links
- Entrez Gene: 9510 Human
- Omim: 605174 Human
- SwissProt: Q9UHI8 Human
- Unigene: 643357 Human
- Unigene: 8230 Human
Anti-ADAMTS1 antibody - Aminoterminal end images:
Western blot - ADAMTS1 antibody - Aminoterminal end (ab28284)
Anti-ADAMTS1 antibody - Aminoterminal end (ab28284) at 1/2500 dilution + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 106 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence-ADAMTS1 antibody - Aminoterminal end(ab28284)
ICC/IF image of ab28284 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28284, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ADAMTS1 antibody - Aminoterminal end(ab28284)
IHC image of ab28284 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28284, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References for Anti-ADAMTS1 antibody - Aminoterminal end (ab28284)
This product has been referenced in:
- Peluffo MCet al. Systematic analysis of protease gene expression in the rhesus macaque ovulatory follicle: metalloproteinase involvement in follicle rupture. Endocrinology 152:3963-74 (2011). IHC-P; Rhesus monkey.Read more (PubMed: 21791558) »
- Keightley MCet al. PGF2a-F-prostanoid receptor signalling via ADAMTS1 modulates epithelial cell invasion and endothelial cell function in endometrial cancer. BMC Cancer 10:488 (2010). IHC-P, Neutralising; Human.Read more (PubMed: 20840749) »
See all 2 publications for this product
Publishing research using ab28284? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"