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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Anti-ADAMTS1 antibody - Carboxyterminal end (ab39194)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab39194? Please let us know so that we can cite the reference in this datasheet
ab39194 has been referenced in 2 publications.
- Keller KE et al. Differential effects of ADAMTS-1, -4, and -5 in the trabecular meshwork. Invest Ophthalmol Vis Sci 50:5769-77 (2009). PubMed: 19553617
- Su SC et al. Molecular profile of endothelial invasion of three-dimensional collagen matrices: insights into angiogenic sprout induction in wound healing. Am J Physiol Cell Physiol : (2008). PubMed: 18787072
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - ADAMTS1 antibody - Carboxyterminal end (ab39194)
ICC/IF image of ab39194 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39194, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS1 antibody - Carboxyterminal end (ab39194)
ab39194 staining ADAMTS1 in Human Placenta (Decidua) tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with a peroxidase block for 5 minutes at room temperature. Antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with primary antibody (1/50) in antibody diluent for 1 hour. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.
This image is courtesy of an Abreview submitted by Dr Hyangsin Lee
ADAMTS1 antibody - Carboxyterminal end for IHC-P in Human (39194)
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