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Immunocytochemistry/ Immunofluorescence-ADAMTS1 antibody - Carboxyterminal end(ab39194)
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Product Name
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ADAMTS1 antibody - Carboxyterminal end
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See all ADAMTS1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADAMTS1 - Carboxyterminal end
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Immunogen
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Synthetic peptide based on the carboxy end of human ADAMTS1.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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Ab39194 recognizes the zymogen of ADAMTS1, and the furin cleaved ADAMTS1, but not the shed form.
We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS1 antibodies
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Epitope
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This antibody recognizes the last 28 amino acids of the protein.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted. Associated with the extracellular matrix.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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ADAMTS1 is a metalloproteinase of the ADAM (A Disintegrin And Metalloproteinase) family containing disintegrin-like domains. ADAMTS1, also known as METH1, was first described as a protein elevated in invasive mouse tumors. Initial findings indicated a role for ADAMTS1 in tumor progression, since the protein was preferentially expressed in more invasive tumor cell lines. ADAMTS1 is necessary for normal growth, fertility, and organ morphology and function.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS1 antibody - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADAMTS1 antibody - Carboxyterminal end images, references, products related to ab39194 and other tools.
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ADAMTS1 antibody - Carboxyterminal end images:
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 Immunocytochemistry/ Immunofluorescence-ADAMTS1 antibody - Carboxyterminal end(ab39194)
ICC/IF image of ab39194 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39194, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for ADAMTS1 antibody - Carboxyterminal end (ab39194)
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This product has been used in:
Su SC et al. Molecular profile of endothelial invasion of three-dimensional collagen matrices: insights into angiogenic sprout induction in wound healing. Am J Physiol Cell Physiol : (2008). PubMed: 18787072
If you publish research using ab39194 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ADAMTS1
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