ADAMTS13 antibody - Carboxyterminal end long isoform (ab28273)

Product Name 

ADAMTS13 antibody - Carboxyterminal end long isoform

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAMTS13 - Carboxyterminal end long isoform

Immunogen 

Synthetic peptide based on the carboxyterminal end of full length human ADAMTS13.(Peptide available as ab41251.)

Reacts with 
(species key)

Hu

Specificity 

ab28273 recognises the Carboxyterminal end of the long form of ADAMTS13.

We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS13 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa). Glycosylation and the abundance of cysteine residues gives ADAMTS13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands between 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family

Relevance 

ADAMTS13, also known as von Willebrands factor cleaving protease (vWFcp), was first identified by its ability to cleave the ultra large aggregates of vWF into smaller forms in the serum. A thrombocytopenic disorder called thrombic thrombocytopenic purpura (TTR) was shown to be due to a deficiency in the cleavage of von Willebrands factor, and ADAMTS13 was identified as the enzyme responsible. ADAMTS13 cleaves the Tyr1605 to Met1606 bond in the A2 domain of vWF, a process stimulated by shear stress in the circulation and by binding to either platelet glycoprotein IBa or to heparin. The cleaved vWF leads to decreased platelet adhesion. An idiopathic form of TTR is found in patients with auto antibodies to ADAMTS13.
The full length ADAMTS-1 sequence codes for a 1,427 amino acid protein, with a predicted mass of 153.6 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels. The variants reported include 1,371, 1,340 and 344 amino acids, with predicted mass of 130.3, 144.5 and 36.7 kDa respectively. Many bands of varying sizes can be seen on Western blots, between 110-190 kDa, perhaps indicating differentially processed ADAMTS-13.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for ADAMTS13 antibody - Carboxyterminal end long isoform images, references, products related to ab28273 and other tools.

ADAMTS13 antibody - Carboxyterminal end long isoform images:

Customer reviews (feedback) regarding ADAMTS13 antibody - Carboxyterminal end long isoform

Customer FAQs regarding ADAMTS13 antibody - Carboxyterminal end long isoform

Protocols for ADAMTS13 antibody - Carboxyterminal end long isoform

Price and availability of products related to ADAMTS13 antibody - Carboxyterminal end long isoform

ADAMTS13 antibody - Carboxyterminal end long isoform - related products: