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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Overview
Product name
Anti-ADAMTS13 antibody - Carboxyterminal end long isoform
See all ADAMTS13 products (8) ...
Description
Rabbit polyclonal to ADAMTS13 - Carboxyterminal end long isoform
Specificity
ab28273 recognises the Carboxyterminal end of the long form of ADAMTS13.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the carboxyterminal end of full length human ADAMTS13. (Peptide available as ab41251.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/1000 - 1/5000.Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa).(1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Detects a band of approximately 190 kDa. Glycosylation and the abundance of cysteine residues gives ADAMTS13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands between 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection.)
Target
Function
Cleaves the vWF multimers in plasma into smaller forms.
Tissue specificity
Plasma. Expressed primarily in liver.
Involvement in disease
Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.
Sequence similarities
Contains 2 CUB domains.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 8 TSP type-1 domains.
Domain
The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.
Post-translational
modifications
May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ76LX8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Von Willebrand factor cleaving protease antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
see all
Database links
- Entrez Gene: 11093 Human
- Omim: 604134 Human
- SwissProt: Q710F6 Human
- SwissProt: Q711T8 Human
- SwissProt: Q76LX8 Human
- SwissProt: Q96L37 Human
- SwissProt: Q9H0G3 Human
- Unigene: 131433 Human
Anti-ADAMTS13 antibody - Carboxyterminal end long isoform images:
Western blot - ADAMTS13 antibody - Carboxyterminal end long isoform (ab28273)
All lanes : Anti-ADAMTS13 antibody - Carboxyterminal end long isoform (ab28273) at 1 µg/ml
Lane 1 : Recombinant Human ATS-13 at 0.08 µg
Lane 2 : Recombinant Human ATS-13 at 0.04 µg
Lane 3 : Recombinant Human ATS-13 at 0.02 µg
Predicted band size : 154 kDa
Glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels.
Immunocytochemistry/ Immunofluorescence-ADAMTS13 antibody - Carboxyterminal end long isoform(ab28273)
ICC/IF image of ab28273 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28273, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-ADAMTS13 antibody - Carboxyterminal end long isoform (ab28273)
ab28273 has not yet been referenced specifically in any publications.
Publishing research using ab28273? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"