Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Overview
Product name
Anti-ADAMTS13 antibody - Metalloproteinase domain
See all ADAMTS13 products (8) ...
Description
Rabbit polyclonal to ADAMTS13 - Metalloproteinase domain
Specificity
ab28274 recognises the metalloproteinase domain of ADAMTS13.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide based on the metalloproteinase domain of human ADAMTS 13. (Peptide available as ab41249.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
-
Western blot - ADAMTS13 antibody - Metalloproteinase domain (ab28274)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa).(1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Glycosylation and the abundance of cysteine residues gives ADAMTS 13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands at 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection.)
Target
Function
Cleaves the vWF multimers in plasma into smaller forms.
Tissue specificity
Plasma. Expressed primarily in liver.
Involvement in disease
Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.
Sequence similarities
Contains 2 CUB domains.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 8 TSP type-1 domains.
Domain
The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.
Post-translational
modifications
May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ76LX8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Von Willebrand factor cleaving protease antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
see all
Database links
- Entrez Gene: 11093 Human
- Entrez Gene: 279028 Mouse
- Entrez Gene: 362091 Rat
- Omim: 604134 Human
- SwissProt: Q710F6 Human
- SwissProt: Q711T8 Human
- SwissProt: Q76LX8 Human
- SwissProt: Q96L37 Human
see all
Anti-ADAMTS13 antibody - Metalloproteinase domain images:
Western blot - ADAMTS13 antibody - Metalloproteinase domain (ab28274)
All lanes : Anti-ADAMTS13 antibody - Metalloproteinase domain (ab28274) at 1 µg/ml
Lane 1 : Recombinant Human ATS-13 at 0.08 µg
Lane 2 : Recombinant Human ATS-13 at 0.04 µg
Lane 3 : Recombinant Human ATS-13 at 0.02 µg
Predicted band size : 154 kDa
Glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels.
Protocols
References for Anti-ADAMTS13 antibody - Metalloproteinase domain (ab28274)
ab28274 has not yet been referenced specifically in any publications.
Publishing research using ab28274? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"