Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Overview
Product name
Anti-ADAMTS15 antibody
See all ADAMTS15 products (3) ...
Description
Rabbit polyclonal to ADAMTS15
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human, Rhesus monkey
Predicted to work with
Mouse
Immunogen
Synthetic peptide: HSTNRLTLAVAW, corresponding to amino acids 592-603 of Human ADAMTS15
HSTNRLTLAV AW
Positive control
293EBNA cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
-
Western blot - ADAMTS15 antibody (ab28516)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 55 kDa (predicted molecular weight: 103 kDa).
IHC-P: Use at an assay dependent concentration. (PubMed: 21791558)
Target
Relevance
ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration.
Cellular localization
Secreted
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 15 antibody
- A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 antibody
- A disintegrin like and metalloprotease Reprolysin type with thrombospondin type 1 motif 15 preproprotein antibody
see all
Database links
- Entrez Gene: 170689 Human
- Entrez Gene: 235130 Mouse
- Omim: 607509 Human
- SwissProt: Q32MI6 Human
- SwissProt: Q8TE58 Human
- SwissProt: Q504Z2 Mouse
- Unigene: 534221 Human
Anti-ADAMTS15 antibody images:
Western blot - ADAMTS15 antibody (ab28516)
Anti-ADAMTS15 antibody (ab28516) at 1/1000 dilution + Supernatant of 293 EBNA cells overexpressing ADAMTS15. at 20 µl
Predicted band size : 103 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
References for Anti-ADAMTS15 antibody (ab28516)
This product has been referenced in:
- Peluffo MCet al. Systematic analysis of protease gene expression in the rhesus macaque ovulatory follicle: metalloproteinase involvement in follicle rupture. Endocrinology 152:3963-74 (2011). IHC-P; Rhesus monkey.Read more (PubMed: 21791558) »
- Pockert AJet al. Modified expression of the ADAMTS enzymes and tissue inhibitor of metalloproteinases 3 during human intervertebral disc degeneration. Arthritis Rheum 60:482-91 (2009). IHC-P; Human.Read more (PubMed: 19180493) »
See all 2 publications for this product
Publishing research using ab28516? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"