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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-ADAMTS2 antibody
See all ADAMTS2 products (6) ...
Description
Rabbit polyclonal to ADAMTS2
Specificity
This antibody is specific for ADAMTS2 (catalytic domain).
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthetic peptide based on the catalytic domain human ADAMTS2, after the first furin cleavage site.
(Peptide available as ab45224.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab44210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 135 kDa. The full length ADAMTS2 gives a greater apparent molecular weight on reduced SDS-PAGE gels due to glycosylation and the abundance of cysteine residues. Purified ADAMTS2 resolves at a lower molecular weight of 107 kDa, due to cleavage at the furin sites. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis.
Tissue specificity
Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain.
Involvement in disease
Defects in ADAMTS2 are the cause of Ehlers-Danlos syndrome type 7C (EDS7C) [MIM:225410]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7C is marked by extremely fragile tissues, hyperextensible skin and easy bruising. Facial skin contains numerous folds, as in the cutis laxa syndrome.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 PLAC domain.
Contains 4 TSP type-1 domains.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionO95450
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 2 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 2 antibody
- ADAM metallopeptidase with thrombospondin type 1 motif 2 antibody
see all
Database links
- Entrez Gene: 9509 Human
- Entrez Gene: 216725 Mouse
- Entrez Gene: 287899 Rat
- Omim: 604539 Human
- SwissProt: O95450 Human
- SwissProt: Q5NCE0 Mouse
- SwissProt: Q8C9W3 Mouse
- Unigene: 23871 Human
see all
Protocols
References for Anti-ADAMTS2 antibody (ab44210)
ab44210 has not yet been referenced specifically in any publications.
Publishing research using ab44210? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"