Anti-ADAMTS3 antibody - Carboxyterminal end (ab39199)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab39199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Predicted molecular weight: 136 kDa. Glycosylation and the abundance of cysteine residues gives ADAMTS3 a greater apparent molecular weight on reduced SDS PAGE gels. Purified ADAMTS3 resolves at a lower molecular weight of 108 kD, due to cleavage at the furin site. This antibody recognizes the zymogen of ADAMTS3 at 140-130 kD in reduced Western blots, activated forms at 105-94 kD (major bands), and breakdown products at 50 kD, 34 kD in cell culture media and lysates. A strong band at about 53 kD is seen in media of some cell types, perhaps an alternative form. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceADAMTS3 is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS3 (A Disintegrin And Metalloproteinase with ThromboSpondin 3 motif was first described in human brain. Human ADAMTS3 is 61% identical to human ADAMTS2. Both ADAMTS2 and ADAMTS3 have the ability to process procollagen II, but it is suggested that ADAMTS3 is the majorprocollagen II N-propeptidase. ADAMTS3 expression is found in placenta, cartilage, skin, lung, brain, and heart. Full length human ADAMTS3 contains 1201 amino acids and has a predicted mass of 135kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS3 a greater apparent molecular weight on reduced SDS-PAGE gels. Purified ADAMTS3 resolves at a lower molecular weight of 108 kDa, due to cleavage at the furin site. ADAMTS3 contains the canonical HexxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving procollagen II. In addition to the metalloprotease domain, ADAMTS3 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, and three thrombospondin-1 like domains, followed by a C-terminal domain unique to ADAMTS2 and ADAMTS3. ADAMTS3 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein, much of which binds to the ECM (extracellular matrix).
    • Cellular localizationSecreted. Associated with the extracellular matrix.
    • Database links
    • Alternative names
      • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 3 antibody
      • KIAA0366 antibody
      • KIAA0366 antibody
      • PC II NP antibody
      • Procollagen II amino propeptide processing enzyme antibody
      • Procollagen II N proteinase antibody
      • Zinc metalloendopeptidase antibody
      • Zinc metalloendopeptidase antibody
      see all

    References for Anti-ADAMTS3 antibody - Carboxyterminal end (ab39199)

    ab39199 has not yet been referenced specifically in any publications.

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