Feedback Form

Product Name 

ADAMTS3 peptide (Propeptide domain)

Product type 

Proteins and Peptides

Peptide 

This peptide was used as an immunogen for ab39200 - ADAMTS3 antibody - Propeptide domain.

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS3 antibodies

Tested applications 
(see key)

ELISA, WB

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab39200. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP

Relevance 

ADAMTS3 is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS3 (A Disintegrin And Metalloproteinase with ThromboSpondin 3 motif was first described in human brain. Human ADAMTS3 is 61% identical to human ADAMTS2. Both ADAMTS2 and ADAMTS3 have the ability to process procollagen II, but it is suggested that ADAMTS3 is the majorprocollagen II N-propeptidase. ADAMTS3 expression is found in placenta, cartilage, skin, lung, brain, and heart. Full length human ADAMTS3 contains 1201 amino acids and has a predicted mass of 135kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS3 a greater apparent molecular weight on reduced SDS-PAGE gels. Purified ADAMTS3 resolves at a lower molecular weight of 108 kDa, due to cleavage at the furin site. ADAMTS3 contains the canonical HexxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving procollagen II. In addition to the metalloprotease domain, ADAMTS3 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, and three thrombospondin-1 like domains, followed by a C-terminal domain unique to ADAMTS2 and ADAMTS3. ADAMTS3 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein, much of which binds to the ECM (extracellular matrix).

 

ADAMTS3 peptide (Propeptide domain) (ab41234)

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS3 peptide (Propeptide domain) is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to ADAMTS3

 

ADAMTS3 peptide (Propeptide domain) - more information

Customer reviews (feedback) regarding ADAMTS3 peptide (Propeptide domain)

Customer FAQs regarding ADAMTS3 peptide (Propeptide domain)

Protocols for ADAMTS3 peptide (Propeptide domain)

Price and availability of products related to ADAMTS3 peptide (Propeptide domain)

 

ADAMTS3 peptide (Propeptide domain) - related products:

Products for Western blot

Loading controls

Lysates

 

Corresponding Antibody for ADAMTS3 peptide (Propeptide domain)

ab39200

  

Rabbit polyclonal to ADAMTS3 - Propeptide domain

 

Other ADAMTS3 Antibodies

See all ADAMTS3 antibodies (5)...

 

ADAMTS3 peptide (Propeptide domain) - other tools:

Contact Abcam with a Technical Enquiry about ADAMTS3 peptide (Propeptide domain)

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.