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Product Name
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ADAMTS3 peptide (Propeptide domain)
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Product type
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Proteins and Peptides
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Peptide
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This peptide was used as an immunogen for ab39200 - ADAMTS3 antibody - Propeptide domain.
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Specificity
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We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS3 antibodies
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Tested applications
(see key)
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ELISA, WB
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab39200. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Relevance
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ADAMTS3 is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS3 (A Disintegrin And Metalloproteinase with ThromboSpondin 3 motif was first described in human brain. Human ADAMTS3 is 61% identical to human ADAMTS2. Both ADAMTS2 and ADAMTS3 have the ability to process procollagen II, but it is suggested that ADAMTS3 is the majorprocollagen II N-propeptidase. ADAMTS3 expression is found in placenta, cartilage, skin, lung, brain, and heart. Full length human ADAMTS3 contains 1201 amino acids and has a predicted mass of 135kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS3 a greater apparent molecular weight on reduced SDS-PAGE gels. Purified ADAMTS3 resolves at a lower molecular weight of 108 kDa, due to cleavage at the furin site. ADAMTS3 contains the canonical HexxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving procollagen II. In addition to the metalloprotease domain, ADAMTS3 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, and three thrombospondin-1 like domains, followed by a C-terminal domain unique to ADAMTS2 and ADAMTS3. ADAMTS3 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein, much of which binds to the ECM (extracellular matrix).
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ADAMTS3 peptide (Propeptide domain) (ab41234) |
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS3 peptide (Propeptide domain) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to ADAMTS3
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ADAMTS3 peptide (Propeptide domain) - more information
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