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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
ADAMTS3 peptide (Propeptide domain)
See all ADAMTS3 products (3) ...
Protein description
This peptide was used as an immunogen for ab39200 - ADAMTS3 antibody - Propeptide domain.
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
Concentration
Concentration information loading...
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab39200. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
ADAMTS3 is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS3 (A Disintegrin And Metalloproteinase with ThromboSpondin 3 motif was first described in human brain. Human ADAMTS3 is 61% identical to human ADAMTS2. Both ADAMTS2 and ADAMTS3 have the ability to process procollagen II, but it is suggested that ADAMTS3 is the majorprocollagen II N-propeptidase. ADAMTS3 expression is found in placenta, cartilage, skin, lung, brain, and heart. Full length human ADAMTS3 contains 1201 amino acids and has a predicted mass of 135kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS3 a greater apparent molecular weight on reduced SDS-PAGE gels. Purified ADAMTS3 resolves at a lower molecular weight of 108 kDa, due to cleavage at the furin site. ADAMTS3 contains the canonical HexxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving procollagen II. In addition to the metalloprotease domain, ADAMTS3 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, and three thrombospondin-1 like domains, followed by a C-terminal domain unique to ADAMTS2 and ADAMTS3. ADAMTS3 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein, much of which binds to the ECM (extracellular matrix).
Cellular localization
Secreted. Associated with the extracellular matrix.
References for ADAMTS3 peptide (Propeptide domain) (ab41234)
ab41234 has not yet been referenced specifically in any publications.
Publishing research using ab41234? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"