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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
Anti-ADAMTS4 antibody - C-terminal
See all ADAMTS4 products (7) ...
Description
Rabbit polyclonal to ADAMTS4 - C-terminal
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
KLH conjugated synthetic peptide selected from the C-terminal region of human ADAMTS4.
Positive control
Mouse cerebellum tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab82300 is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Western blot - ADAMTS4 antibody - C-terminal (ab82300)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Predicted molecular weight: 90 kDa.
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site.
Tissue specificity
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionO75173
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADAMTS 4 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
see all
Database links
- Entrez Gene: 9507 Human
- Entrez Gene: 240913 Mouse
- Omim: 603876 Human
- SwissProt: O75173 Human
- SwissProt: Q8NEK2 Human
- SwissProt: Q8BNJ2 Mouse
- Unigene: 211604 Human
- Unigene: 475917 Mouse
Anti-ADAMTS4 antibody - C-terminal images:
Western blot - ADAMTS4 antibody - C-terminal (ab82300)
Anti-ADAMTS4 antibody - C-terminal (ab82300) at 1/50 dilution + mouse cerebellum tissue lysate at 35 µg
Predicted band size : 90 kDa
Observed band size : 90 kDa
References for Anti-ADAMTS4 antibody - C-terminal (ab82300)
This product has been referenced in:
- Keller KEet al. Differential effects of ADAMTS-1, -4, and -5 in the trabecular meshwork. Invest Ophthalmol Vis Sci 50:5769-77 (2009).Read more (PubMed: 19553617) »
See 1 publication for this product
Publishing research using ab82300? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"