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Western blot - ADAMTS4 antibody (ab84792)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS4 antibody (ab84792)
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Product Name
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ADAMTS4 antibody
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See all ADAMTS4 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADAMTS4
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Immunogen
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Synthetic peptide from the C-terminal of Human ADAMTS4 (NM_005099).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/160000.
IHC-P: 1/100 - 1/500.
WB: 1/200 - 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human brain tissue. Human fetal brain lysates.
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Cellular localization
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Secreted. Associated with the extracellular matrix.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Relevance
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A disintegrin like and metalloproteinase thrombospondin type 1 motif 4 (ADAMTS4 or aggrecanase 1) is a member of the ADAMTS family of multidomain proteins that has demonstrated metalloproteinase functions. The ADAMTS4 protein contains a prohormone convertase cleavage site, thrombospondin 1 like domain, cysteine rich domain, and a propeptide domain. ADAMTS4 activity had been shown to be blocked by TIMP1, 2, 3, and 4.
ADAMTS4 plays an important role in the cleavage of aggrecan, versican and brevican. Studies have determined that aggrecan is cleaved within the interglobular domain of aggrecan at sites Glu(373) Ala(374) and Phe(342) Glu(373). Aggrecan degradation has been linked to the development of rheumatoid arthritis, osteoarthritis, retinal diseases, Alzheimer's and other neurodegenerative diseases.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADAMTS4 antibody images, references, products related to ab84792 and other tools.
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ADAMTS4 antibody images:
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 Western blot - ADAMTS4 antibody (ab84792)
ADAMTS4 antibody (ab84792) at 1/1000 dilution + Human fetal brain lysates
Predicted band size : 90 kDa
Observed band size : 90 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS4 antibody (ab84792)
ab84792 at a 1/200 dilution staining ADAMTS4 in Human brain tissue.
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Search PubMed (MEDLINE) for references to ADAMTS4
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ADAMTS4 antibody - more information
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Customer reviews (feedback) regarding ADAMTS4 antibody |
Customer FAQs regarding ADAMTS4 antibody |
Protocols for ADAMTS4 antibody |
Price and availability of products related to ADAMTS4 antibody |
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ADAMTS4 antibody - related products:
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ADAMTS4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ADAMTS4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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