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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
Anti-ADAMTS4 antibody
See all ADAMTS4 products (7) ...
Description
Rabbit polyclonal to ADAMTS4
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C-terminal of Human ADAMTS4 (NM_005099).
Positive control
Human brain tissue. Human fetal brain lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200 - 1/500.Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
ELISA
ELISA: 1/160000ELISA: 1/160000
IHC-P: 1/100 - 1/500.
Target
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site.
Tissue specificity
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionO75173
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADAMTS 4 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
see all
Database links
- Entrez Gene: 9507 Human
- Omim: 603876 Human
- SwissProt: O75173 Human
- SwissProt: Q8NEK2 Human
- Unigene: 211604 Human
Anti-ADAMTS4 antibody images:
Western blot - ADAMTS4 antibody (ab84792)
Anti-ADAMTS4 antibody (ab84792) at 1/1000 dilution + Human fetal brain lysates
Predicted band size : 90 kDa
Observed band size : 90 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS4 antibody (ab84792)
ab84792 at a 1/200 dilution staining ADAMTS4 in Human brain tissue.
References for Anti-ADAMTS4 antibody (ab84792)
ab84792 has not yet been referenced specifically in any publications.
Publishing research using ab84792? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"