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Anti-ADAMTS5 antibody - Carboxyterminal end (ab39202)

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Overview

Product name

Anti-ADAMTS5 antibody - Carboxyterminal end
See all ADAMTS5 products (6) ...

Description

Rabbit polyclonal to ADAMTS5 - Carboxyterminal end

Specificity

ab39202 recognises the Carboxyterminal end of ADAMTS5.

Tested applications

IHC-P, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide crresponding to aa 850-890 of the carboxyterminal end of human ADAMTS5.

(Peptide available as ab43061.)

Positive control

IHC-P: Human normal liver parenchym

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab39202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.

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    WB

     WB: 1/1000Predicted mole...Read more →

    WB: 1/1000Predicted molecular weight: 102 kDa.(when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. This antibody recognizes the zymogen of ADAMTS5 at 120 kDa in reduced Western blots, activated forms at 73 kDa (major bands), and breakdown products at 50 kDa and 40 kDa in cell lysates. Dilution optimised using Chromogenic detection.)

Target

Function

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.

Tissue specificity

Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.

Sequence similarities

Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains.

Domain

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational
modifications

The precursor is cleaved by a furin endopeptidase.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionQ9UNA0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
  • A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
  • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
  • A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody
  • ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody
  • ADAM TS 11 antibody
  • ADAM TS 5 antibody
  • ADAM TS5 antibody
  • ADAM-TS 11 antibody
  • ADAM-TS 5 antibody
  • ADAM-TS5 antibody
  • ADAMTS 11 antibody
  • ADAMTS 5 antibody
  • ADAMTS-11 antibody
  • ADAMTS-5 antibody
  • ADAMTS11 antibody
  • Adamts5 antibody
  • ADMP 2 antibody
  • ADMP-2 antibody
  • ADMP2 antibody
  • Aggrecanase 2 antibody
  • Aggrecanase-2 antibody
  • ATS5_HUMAN antibody
  • FLJ36738 antibody
  • Implantin antibody
  • ThromboSpondin motif-5 antibody
see all

Anti-ADAMTS5 antibody - Carboxyterminal end images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)

Ab39202 staining Human normal liver parenchym. Staining is localized to cytoplasma and secreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-ADAMTS5 antibody - Carboxyterminal end (ab39202)

ab39202 has not yet been referenced specifically in any publications.

Publishing research using ab39202? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"