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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)
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Product Name 

ADAMTS5 antibody - Carboxyterminal end

 

See all ADAMTS5 antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAMTS5 - Carboxyterminal end

Immunogen 

Synthetic peptide crresponding to aa 850-890 of the carboxyterminal end of human ADAMTS5.

(Peptide available as ab43061.)

Reacts with 
(species key)

Hu

Specificity 

ab39202 recognises the Carboxyterminal end of ADAMTS5.

We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS5 antibodies

Tested applications 
(see key)

IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Predicted molecular weight: 102 kDa. This antibody recognizes the zymogen of ADAMTS5 at 120 kD in reduced Western blots, activated forms at 73 kD (major bands), and breakdown products at 50 kD and 40 kD in cell lysates. Dilution optimised using Chromogenic detection.

IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.



Specific protocols
Recommended WB protocol

Positive control 
(see definition)

IHC-P: Human normal liver parenchym

Cellular localization 

Secreted. Associated with the extracellular matrix.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Aggrecan

Relevance 

ADAMTS5, also known as Implantin or Aggrecanase 2, is a member of the larger family of ADAM (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin-5 motif) was first described as ADAMTS5, a protein elevated in mice during the peri-implantion period. At the same time, another group identified Aggrecanase 11, a protein elevated in arthritic synovium. The name was later changed to ADAMTS5. ADAMTS5 is expressed in human and mouse. It has been found in heart, lung, cervix, uterus, ovary, brain, cartilage, and numerous other tissues, as well as chondroblastoma cell lines. Initial observations indicated a role for ADAMTS5 in aggrecan cleavage and cartilage destruction, especially in arthritis, and potentially a role in embryo implantation. ADAMTS5 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to efficiently cleave Aggrecan. In addition to the metalloprotease domain, ADAMTS5 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, a spacer domain, and two thrombospondin-1 like domains. ADAMTS5 is inhibited by the endogenous MMP inhibitors (TIMP1, 2, 3, and 4) but most efficiently by TIMP3. Unlike many of the ADAMs proteases, ADAMTS5 does not have a transmembrane domain, and is a secreted protein. Full length ADAMTS5 is a 930 amino acid protein with a predicted molecular mass is 101.7 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS5 a greater apparent molecular weight on reduced SDS PAGE gels. When ADAMTS5 is secreted, it is cleaved at the furin cleavage site (predicted molecular mass 73.2 kDa) and then further cleaved to generate a range of smaller forms.

 

Alternative names ADAMTS5 antibody - Carboxyterminal end (ab39202)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

11096    (Human)

Omim

    

605007    (Human)

SwissProt

    

Q9UNA0    (Human)

Unigene

    

58324    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS5 antibody - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance!


See below for ADAMTS5 antibody - Carboxyterminal end images, references, products related to ab39202 and other tools.

 

ADAMTS5 antibody - Carboxyterminal end images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)

Ab39202 staining Human normal liver parenchym. Staining is localized to cytoplasma and secreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

 
 

Search PubMed (MEDLINE) for references to ADAMTS5

 

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ADAMTS5 antibody - Carboxyterminal end - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

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Lysates

 

Immunizing Peptide for ADAMTS5 antibody - Carboxyterminal end

ab43061

  

ADAMTS5 peptide (Carboxyterminal end)

 

Compatible Secondaries for ADAMTS5 antibody - Carboxyterminal end

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

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HRP antibody

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Alkaline Phosphatase antibody

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Texas Red ® antibody

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Phycoerythrin antibody

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Agarose antibody

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10nm Gold antibody

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15nm Gold antibody

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20nm Gold antibody

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10nm Gold antibody

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5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

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Chromeo™ 494 antibody

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Chromeo™ 546 antibody

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Chromeo™ 642 antibody

 

Other ADAMTS5 Antibodies

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ADAMTS5 antibody - Carboxyterminal end - other tools:

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