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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Aggrecan
Overview
Product name
Anti-ADAMTS5 antibody - Carboxyterminal end
See all ADAMTS5 products (6) ...
Description
Rabbit polyclonal to ADAMTS5 - Carboxyterminal end
Specificity
ab39202 recognises the Carboxyterminal end of ADAMTS5.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide crresponding to aa 850-890 of the carboxyterminal end of human ADAMTS5.
(Peptide available as ab43061.)
Positive control
IHC-P: Human normal liver parenchym
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Aggrecan
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/1000Predicted molecular weight: 102 kDa.(when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. This antibody recognizes the zymogen of ADAMTS5 at 120 kDa in reduced Western blots, activated forms at 73 kDa (major bands), and breakdown products at 50 kDa and 40 kDa in cell lysates. Dilution optimised using Chromogenic detection.)
Target
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Tissue specificity
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ9UNA0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
see all
Database links
- Entrez Gene: 11096 Human
- Omim: 605007 Human
- SwissProt: Q9UNA0 Human
- Unigene: 58324 Human
Anti-ADAMTS5 antibody - Carboxyterminal end images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody - Carboxyterminal end (ab39202)
Ab39202 staining Human normal liver parenchym. Staining is localized to cytoplasma and secreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References for Anti-ADAMTS5 antibody - Carboxyterminal end (ab39202)
ab39202 has not yet been referenced specifically in any publications.
Publishing research using ab39202? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"