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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Aggrecan
Overview
Product name
Anti-ADAMTS5 antibody
See all ADAMTS5 products (6) ...
Description
Rabbit polyclonal to ADAMTS5
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human ADAMTS5.
(Peptide available as ab41036.)
Positive control
ab41037 gave a positive signal in the following whole cell lysates: Uterus (Human)- normal tissue HeLa (Human epithelial carcinoma cell line) Placenta (Mouse) - normal tissue NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) ab41037 gave a positive signal in the following tissue lysates: Brain (Mouse) Liver (Mouse) Testis (Mouse) Spinal Cord (Mouse) Ovary (Mouse) Brain (Rat) Liver (Rat)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Aggrecan
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250Detects a band of approximately 74 kDa (predicted molecular weight: 102 kDa).
IHC-P: Use a concentration of 10 µg/mlPerform enzymatic antigen retrieval before commencing with IHC staining protocol.
Target
Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Tissue specificity
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains.
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ9UNA0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
see all
Database links
- Entrez Gene: 11096 Human
- Entrez Gene: 23794 Mouse
- Entrez Gene: 304135 Rat
- Omim: 605007 Human
- SwissProt: Q9UNA0 Human
- SwissProt: Q9R001 Mouse
- Unigene: 58324 Human
- Unigene: 112933 Mouse
see all
Anti-ADAMTS5 antibody images:
Western blot - ADAMTS5 antibody (ab41037)
All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
Lane 2 : Placenta (Mouse) Whole Cell Lysate - normal tissue (ab29139)
Lane 3 : Uterus (Human) Whole Cell Lysate - normal tissue
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 102 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.
Western blot - ADAMTS5 antibody (ab41037)
All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : Spinal Cord (Mouse) Tissue Lysate
Lane 7 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 9 : Brain (Rat) Tissue Lysate - normal tissue
Lane 10 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 102 kDa
Observed band size : 74 kDa (why is the actual band size different from the predicted?)
ab41037 appears to detect a 74 kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAMTS5 antibody (ab41037)
ab41037 staining ADAMTS5 in mouse knee joint tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and an enzymatic mediated antigen retrieval step was performed using Proteinase K. Samples were then blocked with 5% serum for 10 minutes at room temperature followed by incubation with the primary antibody at 10µg/ml for 1 hour. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.
This image is a courtesy of an anonymous Abreview
References for Anti-ADAMTS5 antibody (ab41037)
This product has been referenced in:
- Wang Jet al. TNF-a and IL-1ß Promote a Disintegrin-like and Metalloprotease with Thrombospondin Type I Motif-5-mediated Aggrecan Degradation through Syndecan-4 in Intervertebral Disc. J Biol Chem 286:39738-49 (2011). WB; Human, Rat.Read more (PubMed: 21949132) »
- Keller KEet al. Differential effects of ADAMTS-1, -4, and -5 in the trabecular meshwork. Invest Ophthalmol Vis Sci 50:5769-77 (2009).Read more (PubMed: 19553617) »
See all 2 publications for this product
Publishing research using ab41037? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"