Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Overview
Product name
Anti-ADAMTS7 antibody - Propeptide domain
See all ADAMTS7 products (3) ...
Description
Rabbit polyclonal to ADAMTS7 - Propeptide domain
Specificity
This antibody recognizes latent ADAMTS7, and the shed propeptide domain (ADAMTS7 is cleaved at the furin cleavage site when it is secreted).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the propeptide domain of Human ADAMTS7 between amino acids 50-100.
(Peptide available as ab41241.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody has been peptide affinity purified and concentrated.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight for the full length ADAMTS7 sequence is 184 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS7 a greater apparent molecular weight of around 300 kDa on reduced SDS PAGE gels. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS7 is still poorly understood. ADAMTS7 most resembles ADAMTS12 in overall structure. ADAMTS7 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein.
Cellular localization
Secreted protein; extracellular space; extracellular matrix.
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
- ADAM metallopeptidase with thrombospondin type 1 motif 7 antibody
- A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
see all
Database links
- Entrez Gene: 11173 Human
- Omim: 605009 Human
- SwissProt: Q6P7J9 Human
- SwissProt: Q9UKP4 Human
- Unigene: 16441 Human
Protocols
References for Anti-ADAMTS7 antibody - Propeptide domain (ab39891)
ab39891 has not yet been referenced specifically in any publications.
Publishing research using ab39891? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"