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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Overview
Product name
Anti-ADAMTS7 antibody - Propeptide domain
See all ADAMTS7 products (3) ...
Description
Rabbit polyclonal to ADAMTS7 - Propeptide domain
Specificity
This antibody is specific for the prpetide domain after the second furin cleavage site of the full lenght ADAMTS7 and of the short form ADAMTS7A.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the propeptide domain of human ADAMTS7, after the second furin cleavage site between amino acids 80-140.
(Peptide available as ab45240.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 0.5M Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
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Western blot - ADAMTS7 antibody - Propeptide domain (ab45044)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 300kDa (predicted molecular weight: 181 kDa). Glycosylation and the abundance of cysteine residues gives ADAMTS7 a greater apparent molecular weight of around 300kDa on reduced SDS-PAGE gels. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS7 is still poorly understood. ADAMTS7 most resembles ADAMTS12 in overall structure. ADAMTS7 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein.
Cellular localization
Secreted protein; extracellular space; extracellular matrix.
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
- ADAM metallopeptidase with thrombospondin type 1 motif 7 antibody
- A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
see all
Database links
- Entrez Gene: 11173 Human
- Omim: 605009 Human
- SwissProt: Q6P7J9 Human
- SwissProt: Q9UKP4 Human
- Unigene: 16441 Human
Anti-ADAMTS7 antibody - Propeptide domain images:
Western blot - ADAMTS7 antibody - Propeptide domain (ab45044)
All lanes : Anti-ADAMTS7 antibody - Propeptide domain (ab45044) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 35 µl
Lane 2 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 30 µl
Lane 3 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 25 µl
Predicted band size : 181 kDa
Protocols
References for Anti-ADAMTS7 antibody - Propeptide domain (ab45044)
ab45044 has not yet been referenced specifically in any publications.
Publishing research using ab45044? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"