|
|
|
|
Western blot - ADAMTS7 antibody - Propeptide domain (ab45044)
(enlarge)
|
|
|
|
|
Product Name
|
ADAMTS7 antibody - Propeptide domain
|
| |
See all ADAMTS7 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ADAMTS7 - Propeptide domain
|
|
Immunogen
|
Synthetic peptide based on the propeptide domain of human ADAMTS7, after the second furin cleavage site between amino acids 80-140.
(Peptide available as ab45240.)
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
This antibody is specific for the prpetide domain after the second furin cleavage site of the full lenght ADAMTS7 and of the short form ADAMTS7A.
We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS7 antibodies
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 300kDa (predicted molecular weight: 181 kDa). Glycosylation and the abundance of cysteine residues gives ADAMTS7 a greater apparent molecular weight of around 300kDa on reduced SDS-PAGE gels. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Secreted protein; extracellular space; extracellular matrix.
|
|
Research areas
|
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
|
|
Relevance
|
ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS7 is still poorly understood. ADAMTS7 most resembles ADAMTS12 in overall structure. ADAMTS7 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein.
|
|
|
ADAMTS7 antibody - Propeptide domain (ab45044) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 0.5M Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS7 antibody - Propeptide domain is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ADAMTS7 antibody - Propeptide domain images, references, products related to ab45044 and other tools.
|
| |
ADAMTS7 antibody - Propeptide domain images:
|
 Western blot - ADAMTS7 antibody - Propeptide domain (ab45044)
All lanes : ADAMTS7 antibody - Propeptide domain (ab45044) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 35 µl
Lane 2 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 30 µl
Lane 3 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 25 µl
Predicted band size : 181 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to ADAMTS7
|
| |
ADAMTS7 antibody - Propeptide domain - more information
|
Customer reviews (feedback) regarding ADAMTS7 antibody - Propeptide domain |
Customer FAQs regarding ADAMTS7 antibody - Propeptide domain |
Protocols for ADAMTS7 antibody - Propeptide domain |
Price and availability of products related to ADAMTS7 antibody - Propeptide domain |
| |
ADAMTS7 antibody - Propeptide domain - related products:
|
|
|
| |
ADAMTS7 antibody - Propeptide domain - other tools:
|
Contact Abcam with a Technical Enquiry about ADAMTS7 antibody - Propeptide domain |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
