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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Overview
Product name
Anti-ADAMTS8 antibody - Amino end of propeptide domain
See all ADAMTS8 products (5) ...
Description
Rabbit polyclonal to ADAMTS8 - Amino end of propeptide domain
Specificity
This antibody recognizes the zymogen of ADAMTS8 at 110 kDa in reduced Western blots, the truncated form at 67 kDa (major band), and breakdown products at 39 kDa in cell lysates.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the aminoterminal end of the propeptide domain of Human ADAMTS8.
(Peptide available as ab45241.)
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 - 1/5000. Predicted molecular weight: 97 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS8 is still poorly understood. ADAMTS8 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active on a range of substrates. ADAMTS8 is inhibited by the endogenous MMP inhibitors, TIMP1, 2, 3 and 4, but most efficiently by TIMP3. In addition to the metalloprotease domain, ADAMTS8 has a propeptide domain, a Prohormone Convertase (PC, furin) cleavage site, a cysteine rich domain and thrombospondin 1 like domains.
Cellular localization
Secreted protein; extracellular space; extracellular matrix.
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 8 antibody
- ADAMTS 8 antibody
- METH 2 antibody
see all
Database links
- Entrez Gene: 11095 Human
- Omim: 605175 Human
- SwissProt: Q9UP79 Human
- Unigene: 271605 Human
Protocols
References for Anti-ADAMTS8 antibody - Amino end of propeptide domain (ab45045)
ab45045 has not yet been referenced specifically in any publications.
Publishing research using ab45045? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"