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Anti-ADAMTS8 antibody - Amino end of propeptide domain (ab45045)

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Overview

Product name

Anti-ADAMTS8 antibody - Amino end of propeptide domain
See all ADAMTS8 products (5) ...

Description

Rabbit polyclonal to ADAMTS8 - Amino end of propeptide domain

Specificity

This antibody recognizes the zymogen of ADAMTS8 at 110 kDa in reduced Western blots, the truncated form at 67 kDa (major band), and breakdown products at 39 kDa in cell lysates.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide based on the aminoterminal end of the propeptide domain of Human ADAMTS8.

(Peptide available as ab45241.)

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab45045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: 1/1000 - 1/5000. Predicted molecular weight: 97 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS8 is still poorly understood. ADAMTS8 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active on a range of substrates. ADAMTS8 is inhibited by the endogenous MMP inhibitors, TIMP1, 2, 3 and 4, but most efficiently by TIMP3. In addition to the metalloprotease domain, ADAMTS8 has a propeptide domain, a Prohormone Convertase (PC, furin) cleavage site, a cysteine rich domain and thrombospondin 1 like domains.

Cellular localization

Secreted protein; extracellular space; extracellular matrix.

Alternative names

  • A disintegrin and metalloproteinase with thrombospondin motifs 8 antibody
  • ADAMTS 8 antibody
  • METH 2 antibody
  • METH 8 antibody
  • METH8 antibody
  • A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 8 antibody
  • A disintegrin like and metalloprotease with thrombospondin type 1 motif 8 antibody
  • ADAM metallopeptidase with thrombospondin type 1 motif 8 antibody
  • ADAM TS 8 antibody
  • ADAM TS8 antibody
  • FLJ41712 antibody
  • METH2 antibody
see all

References for Anti-ADAMTS8 antibody - Amino end of propeptide domain (ab45045)

ab45045 has not yet been referenced specifically in any publications.

Publishing research using ab45045? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"