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Product Name 

ADAMTS8 antibody - Amino end of propeptide domain

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAMTS8 - Amino end of propeptide domain

Immunogen 

Synthetic peptide based on the aminoterminal end of the propeptide domain of Human ADAMTS8.

(Peptide available as ab45241.)

Reacts with 
(species key)

Hu

Specificity 

This antibody recognizes the zymogen of ADAMTS8 at 110 kDa in reduced Western blots, the truncated form at 67 kDa (major band), and breakdown products at 39 kDa in cell lysates.

We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS8 antibodies

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Predicted molecular weight: 97 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.



Specific protocols
Recommended WB protocol

Cellular localization 

Secreted protein; extracellular space; extracellular matrix.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family

Relevance 

ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration.

A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS8 is still poorly understood. ADAMTS8 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active on a range of substrates. ADAMTS8 is inhibited by the endogenous MMP inhibitors, TIMP1, 2, 3 and 4, but most efficiently by TIMP3. In addition to the metalloprotease domain, ADAMTS8 has a propeptide domain, a Prohormone Convertase (PC, furin) cleavage site, a cysteine rich domain and thrombospondin 1 like domains.

 

Alternative names ADAMTS8 antibody - Amino end of propeptide domain (ab45045)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

11095    (Human)

Omim

    

605175    (Human)

SwissProt

    

Q9UP79    (Human)

Unigene

    

271605    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS8 antibody - Amino end of propeptide domain is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to ADAMTS8

 

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Lysates

 

Immunizing Peptide for ADAMTS8 antibody - Amino end of propeptide domain

ab45241

  

ADAMTS8 peptide (Amino end of propeptide domain)

 

Compatible Secondaries for ADAMTS8 antibody - Amino end of propeptide domain

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other ADAMTS8 Antibodies

See all ADAMTS8 antibodies (5)...

 

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