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Western blot - ADAMTS8 antibody - Catalytic domain (ab59822)
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Product Name
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ADAMTS8 antibody - Catalytic domain
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See all ADAMTS8 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADAMTS8 - Catalytic domain
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Immunogen
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A synthetic peptide based on the catalytic domain of human ADAMTS8.
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Reacts with
(species key)
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Hu
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Specificity
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We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS8 antibodies
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa).
A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted protein; extracellular space; extracellular matrix.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAM TS
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
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Relevance
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ADAMTS proteases are secreted enzymes containing a prometalloprotease domain of the reprolysin type. The ADAMTS proteases function in processing of procollagens and von Willebrand factor as well as catabolism of aggrecan, versican and brevican. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration.
A member of the metalloproteinase family containing disintegrin like domains (ADAMs), the function of ADAMTS8 is still poorly understood. ADAMTS8 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active on a range of substrates. ADAMTS8 is inhibited by the endogenous MMP inhibitors, TIMP1, 2, 3 and 4, but most efficiently by TIMP3. In addition to the metalloprotease domain, ADAMTS8 has a propeptide domain, a Prohormone Convertase (PC, furin) cleavage site, a cysteine rich domain and thrombospondin 1 like domains.
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ADAMTS8 antibody - Catalytic domain (ab59822) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAMTS8 antibody - Catalytic domain is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADAMTS8 antibody - Catalytic domain images, references, products related to ab59822 and other tools.
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ADAMTS8 antibody - Catalytic domain images:
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 Western blot - ADAMTS8 antibody - Catalytic domain (ab59822)
All lanes : ADAMTS8 antibody - Catalytic domain (ab59822) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from human epithelial carcinoma A-431 cells (treated with SF)
Lane 2 : Whole cell lysate prepared from human epithelial carcinoma A-431 cells (treated with TPA)
Lane 3 : Whole cell lysate prepared from human epithelial carcinoma A-431 cells (treated with IL1-beta)
Lysates/proteins at 20 µl per lane.
Predicted band size : 97 kDa
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Search PubMed (MEDLINE) for references to ADAMTS8
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ADAMTS8 antibody - Catalytic domain - more information
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