Anti-ADAMTS9 antibody - Propeptide domain (ab28279)

No bands in Western blot with this antibody. Would like ab28276 as a replacement.

ANSWER:

Thank you for your call today and for letting us know about the problem with this antibody.

As we discussed, I'm sending a free of charge vial of ab28276 on the order ***. We are out of stock in our US office, so this vial will be coming from our UK office and we expect it to arrive late next week. Please keep me updated about the results using this antibody.

If you have any data for ab28279 or any protocol information that you would like to forward, it would be very helpful for our investigation into this antibody.

Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

Sorry for the delay, however I was testing antibodies from another
company that ended up giving us the appropriate band and great signal
using both chemiluminescence and fluorescence secondaries.
I was wondering whether it would be alright to go ahead and receive a
refund.
Sorry for the trouble, but your product did not work for us and
i've tried several boiling temperature, gel concentrations,
dilution and incubation times.

ANSWER:

According to our records, we were in touch with regarding ab28279in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more information from you.

For ab28279, I am not able to issue a credit or refund as this antibody was received for free using a testing discount code. Thus, in this case I am only able to offer a free of charge replacement for an antibody of your choice. Please let me know which antibody I can send you free of charge.

If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

We wish you the best of luck with your research and look forward to a reply.

Sorry for the delay, however I was testing antibodies from another
company that ended up giving us the appropriate band and great signal
using both chemiluminescence and fluorescence secondaries.
I was wondering whether it would be alright to go ahead and receive a
refund.
Sorry for the trouble, but your product did not work for us and
i've tried several boiling temperature, gel concentrations,
dilution and incubation times.

ANSWER:

Thank you for your reply.
I am sorry that these antibodies did not perform as stated on the datasheet.

Your credit note ID for the refund of ab39186 is xxx.

As for ab28279, I am not able to issue a credit or refund as this antibody was received for free using a testing discount code. Thus, in this case I am only able to offer a free of charge replacement for an antibody of your choice.Please let me know which antibody I can send you free of charge.

As for the refund, if payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

I look forward to hear back from you.

the answers to your questions
1. the antibody dilution was 1:2000 and the incubation time was 1hr.
2. the blocking reagent: Odyssey-Blocking Buffer
3. yes.
4. there are multiple bands throughout the lanes. nothing stands out
in terms of differences between mice and humans.
5. for both antibodies, i do not see the correct (˜180kDa) band size
and i see multiple others. in the case of ab39186, there are only a
few bands and they are faint (as seen in the image sent with the
abreview). in the case of ab28279, there are several strong bands of
incorrect sizes.

ANSWER:

Thank you for your detailed response.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details for each antibody, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and I'd be happyto offer you a free of charge replacement, credit note, or refund in compensation - for each antibody.

Pleaselet me know how you would like to proceed.
I look forwardhearing back from you and resolving this case.

human breast cancer and mouse samples
mouse is untested
sees bands, but not at the right size
Which protocol, samples were used?
Is an image available?
How much protein was loaded?

ANSWER:

Thank you for your patience.

Unfortunately, I have not yet heard back from the supplying lab. I am really sorry about this, and that I have no specific protocol or WB image to give you. I have emailed them again and will let you know if I receive a reply.

Could you please let me know the following regarding your protocol:

1) What antibody dilution and incubation time was used?
2) What was the blocking reagent?
3) Is the secondary antibody working well with other primary antibodies?
4) Were do you see the bands (which MW) in the human samples and the mouse samples?
5) Is the issue of incorrect band size true forboth antibodies, ab28279 and ab39186?
6) Also, please let me know your order or PO number.

I look forward to hear back from you and resolve the problem for you.