Anti-ADAMTS9 antibody - Spacer between TS1 and TS2 (ab39186)

Sorry for the delay, however I was testing antibodies from another
company that ended up giving us the appropriate band and great signal
using both chemiluminescence and fluorescence secondaries.
I was wondering whether it would be alright to go ahead and receive a
refund.
Sorry for the trouble, but your product did not work for us and
i've tried several boiling temperature, gel concentrations,
dilution and incubation times.

ANSWER:

Thank you for your reply.
I am sorry that these antibodies did not perform as stated on the datasheet.

Your credit note ID for the refund of ab39186 is xxx.

As for ab28279, I am not able to issue a credit or refund as this antibody was received for free using a testing discount code. Thus, in this case I am only able to offer a free of charge replacement for an antibody of your choice.Please let me know which antibody I can send you free of charge.

As for the refund, if payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

I look forward to hear back from you.

the answers to your questions
1. the antibody dilution was 1:2000 and the incubation time was 1hr.
2. the blocking reagent: Odyssey-Blocking Buffer
3. yes.
4. there are multiple bands throughout the lanes. nothing stands out
in terms of differences between mice and humans.
5. for both antibodies, i do not see the correct (˜180kDa) band size
and i see multiple others. in the case of ab39186, there are only a
few bands and they are faint (as seen in the image sent with the
abreview). in the case of ab28279, there are several strong bands of
incorrect sizes.

ANSWER:

Thank you for your detailed response.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details for each antibody, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and I'd be happyto offer you a free of charge replacement, credit note, or refund in compensation - for each antibody.

Pleaselet me know how you would like to proceed.
I look forwardhearing back from you and resolving this case.

human breast cancer and mouse samples
mouse is untested
sees bands, but not at the right size
Which protocol, samples were used?
Is an image available?
How much protein was loaded?

ANSWER:

Thank you for your patience.

Unfortunately, I have not yet heard back from the supplying lab. I am really sorry about this, and that I have no specific protocol or WB image to give you. I have emailed them again and will let you know if I receive a reply.

Could you please let me know the following regarding your protocol:

1) What antibody dilution and incubation time was used?
2) What was the blocking reagent?
3) Is the secondary antibody working well with other primary antibodies?
4) Were do you see the bands (which MW) in the human samples and the mouse samples?
5) Is the issue of incorrect band size true forboth antibodies, ab28279 and ab39186?
6) Also, please let me know your order or PO number.

I look forward to hear back from you and resolve the problem for you.

Please send a discount code for testing this antibody on a previously untested species, mouse.

ANSWER:

DISCOUNT CODE: ******* Expiration date: April 8, 2012

I am very pleased to hear you would like to accept our offer and test ab39168 in mouse. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the results. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.