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Overview

  • Product nameADAMTSL3 protein (His-DHFR tag)
  • Protein descriptionRecombinant fragment corresponding to amino acids 529-788 of Human ADAMTSL3 with N terminal His-DHFR tag; MWt 28.9 kDa excluding tag (BC128389).
  • Uniprot accessionP82987
  • Molecular weight28.900kDa
  • Protein length260 amino acids
  • Expression hostE. coli

    • Properties

  • Purification notesPurity determined to be 80% by SDS-PAGE.
  • FormLyophilised:Reconstitute with water to desired concentration.
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferConstituents: 0.32% Tris HCl, 0.58% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab127140 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        A disintegrin like and metalloprotease domain with thrombospondin type I motifs like 3ADAMTS like 3ADAMTS-like protein 3
        ADAMTSL-3ADAMTSL3ATL3_HUMANPunctin-2
      see all
  • Tissue specificityExpressed in epithelial cells of the colon, fallopian tube, skin, breast, prostate, epididymis, liver, pancreatic islets and bile ducts, as well as by vascular endothelial cells, smooth muscle cells, fibroblasts, cortical and ganglionic neurons and cardiac myocytes. Also expressed by malignant epithelial cells in colon cancer, as well as breast, prostate, renal and skin tumors. Expression is significantly reduced in colon cancer compared to normal colon.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 PLAC domain.
    Contains 10 TSP type-1 domains.
  • Post-translational
    modifications    Glycosylated (By similarity). Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P82987 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for ADAMTSL3 protein (His-DHFR tag) (ab127140)

    ab127140 has not yet been referenced specifically in any publications.

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