Anti-ADAT1 antibody (ab235341)
Key features and details
- Rabbit polyclonal to ADAT1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ADAT1 antibody
See all ADAT1 primary antibodies -
Description
Rabbit polyclonal to ADAT1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Recombinant fragment corresponding to Human ADAT1 aa 1 to the C-terminus.
Database link: Q9BUB4 -
Positive control
- WB: Mouse kidney and liver tissue lysates. THP-1, MD-MBA-231 and HL-60 whole cell lysates. IHC-P: Human skin and tonsil tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
|
|
WB |
1/500 - 1/2000. Predicted molecular weight: 55 kDa.
|
Notes |
---|
IHC-P
1/20 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 55 kDa. |
Target
-
Function
Specifically deaminates adenosine-37 to inosine in tRNA-Ala. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the ADAT1 family.
Contains 1 A to I editase domain. - Information by UniProt
-
Database links
- Entrez Gene: 101925428 Cynomolgus monkey
- Entrez Gene: 23536 Human
- Entrez Gene: 30947 Mouse
- Omim: 604230 Human
- SwissProt: Q4R7N3 Cynomolgus monkey
- SwissProt: Q9BUB4 Human
- SwissProt: Q9JHI2 Mouse
- Unigene: 729312 Human
see all -
Alternative names
- ADAT 1 antibody
- ADAT1 antibody
- ADAT1_HUMAN antibody
see all
Images
-
All lanes : Anti-ADAT1 antibody (ab235341) at 1/500 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 55 kDa -
Paraffin-embedded human skin tissue stained for ADAT1 with ab235341 at 1/100 dilution in immunohistochemical analysis.
-
Paraffin-embedded human tonsil tissue stained for ADAT1 with ab235341 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab235341 has not yet been referenced specifically in any publications.