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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Overview
Product name
Anti-ADAT1 antibody
Description
Rabbit polyclonal to ADAT1
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide: AISWSAVPEQ PLDVTANGFP QGTTKKTIGS LQARSQISKV ELFRSFQKLL, corresponding to amino acids 397-446 of Human ADAT1
AISWSAVPEQ PLDVTANGFP QGTTKKTIGS LQARSQISKV ELFRSFQKLL
Positive control
Jurkat cell lysate and Human heart tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
ELISA: Use at an assay dependent dilution.
Target
Function
Specifically deaminates adenosine-37 to inosine in tRNA-Ala.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the ADAT1 family.
Contains 1 A to I editase domain.
Target information above from: UniProt accessionQ9BUB4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADAT 1 antibody
- ADAT1 antibody
- ADAT1_HUMAN antibody
see all
Database links
- Entrez Gene: 23536 Human
- Entrez Gene: 30947 Mouse
- Entrez Gene: 690810 Rat
- Omim: 604230 Human
- SwissProt: Q9BUB4 Human
- SwissProt: Q9JHI2 Mouse
- Unigene: 729312 Human
- Unigene: 282162 Mouse
Anti-ADAT1 antibody images:
Western blot - ADAT1 antibody (ab50994)
Lane 1 : MW marker
Lane 2 : Anti-ADAT1 antibody (ab50994) at 1.25 µg/ml
Lane 1 : As above
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 39 kDa
Observed band size : 39 kDa
Gel concentration: 12%
Immunohistochemistry (Paraffin-embedded sections) - ADAT1 antibody (ab50994)
ab50994 staining of Paraffin embeded Human heart tissue in IHC application at 4-8 ug/ml dilution. Cells with positive label (Myocardial cells) are indicated with arrows. Magnification: 400x
References for Anti-ADAT1 antibody (ab50994)
ab50994 has not yet been referenced specifically in any publications.
Publishing research using ab50994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"