Overview
Product nameADBR2 + Giα3 fusion protein (Active)
Protein descriptionRecombinant full length Human ADBR2 (amino acids 1-413; UniProt P07550) with N terminal FLAG tag and C terminal His tag, coexpressed with Human Gia3 (UniProt P08754) in Sf9 insect cells.
Expression hostBaculovirus infected sf9 cells
Properties
Purification notesMembrane suspension.
Biological activityActivity: 7.6 pmol/mg.
FormLiquid
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 75mM Tris HCl, 12.5mM Magnesium chloride, 1mM EDTA, pH 7.4
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab90750
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| Functional Studies |
|
Application notesFuncS: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
-
Alternative names
Adrenergic beta 2 receptor surfaceB2ARBARbeta 2 adrenoceptorBeta 2 adrenoreceptorBETA2ARCatecholamine receptorG(i) alpha 3Guanine nucleotide binding protein G(k) subunit alpha
see all
RelevanceADBR2 is a member of the G protein-coupled receptor superfamily. It is activated by the catecholamine epinephrine and couples to the G protein Gs to mediate adenylate cyclase activation. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Gia3 (GNAI3) is the alpha subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase once its G protein coupled receptors (GPCR) such as a2 adrenergic receptors are activated. GNAI3 can be inactivated by pertussis toxin. Guanine nucleotide binding proteins are involved as modulators or transducers in various transmembrane signaling systems. Gia3 is the stimulatory G protein of receptor-regulated K+ channels.
Cellular localizationADBR2: Cell Membrane
References for ADBR2 + Giα3 fusion protein (Active) (ab90750)
ab90750
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
