Anti-AC3 antibody - C-terminal (ab199157)
Key features and details
- Rabbit polyclonal to AC3 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AC3 antibody - C-terminal
See all AC3 primary antibodies -
Description
Rabbit polyclonal to AC3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human AC3 (C terminal). BC126235
Database link: O60266 -
Positive control
- WB: Raji and T98G cell lysate, Mouse and rat brain tissue lysate IHC-P: Human colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/200 - 1/1000. Predicted molecular weight: 129 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 129 kDa. |
Target
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Function
Mediates odorant detection (possibly) via modulation of intracellular cAMP concentration. -
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. -
Sequence similarities
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 2 guanylate cyclase domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 109 Human
- Entrez Gene: 104111 Mouse
- Entrez Gene: 64508 Rat
- Omim: 600291 Human
- SwissProt: O60266 Human
- SwissProt: Q8VHH7 Mouse
- SwissProt: P21932 Rat
- Unigene: 727540 Human
see all -
Alternative names
- AC-III antibody
- AC3 antibody
- ADCY3 antibody
see all
Images
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All lanes : Anti-AC3 antibody - C-terminal (ab199157) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : T98G cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution
Predicted band size: 129 kDa
Exposure time: 30 secondsGel: 6% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling AC3 with ab199157 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab199157 has been referenced in 1 publication.
- Zou J et al. LINC00319 acts as a microRNA-335-5p sponge to accelerate tumor growth and metastasis in gastric cancer by upregulating ADCY3. Am J Physiol Gastrointest Liver Physiol 318:G10-G22 (2020). PubMed: 31433213