ADCY3 antibody (ab14778)

Product Name 

ADCY3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADCY3

Immunogen 

The antibodies were generated against a synthetic peptide sequence (unfortunately, the amino acid sequence is considered to be commercially sensitive) unique to PAC3 protein. All peptides were amidated before conjugation.

Reacts with 
(species key)

Hu, Ms, Rat, Dog

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: Use 5 ul for 2 x 10⁷ cells.
WB: 1/500. Detects a band of approximately 119 kDa (predicted molecular weight: 129 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Integral membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR

Relevance 

The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors (GPCRs). Using the high inter-species homology of mammalian AC isoforms, nine Adenylyl cyclase (AC) isoforms, encoded by separate genes, have been identified until today. Human adenylate cyclase genes comprise of 11 to 26 exons, which are distributed over 16 to 430kb. The expression profile of these 9 AC isoforms in a panel of 16 human tissues and in human embryonic kidney (HEK) cells have been demonstrated earlier. The cAMP synthesizing enzymes are found in two forms: cytosolic (soluble) and membrane-bound (particulate). Stimulation of adenylate cyclases produce cAMP form ATP in response to the activation of GPCRs by various hormones, neurotransmitters and other regulatory molecules. cAMP, in subsequent steps down the signal transduction pathway, can stimulate cAMP-dependent protein kinase A (cPKA), and several other target molecules. Activation of cPKA can phosphorylate a broad range of substrates that regulate various metabolic pathways, gene expression, and affect memory functions etc. The stimulation of adenylate cyclases starts with interactions with GPCRs mediated signals initiated by Gs and Gi heterotrimeric G-proteins. The interaction of GPCR agonist (eg. Interaction of isopreternol to beta2 receptors) catalyses the exchange of GDP to GTP that is bound to G proteins. The GTP binding reduces the affinity of GsĄ to other GTP binding proteins and Gs-GTP complex stimulate the adenylate cyclase. In last several years, new members of particulate and soluble adenylate cyclase family have been identified and significant progress is made in understanding of the molecular mechanisms that underlie the regulation of these families of enzymes.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

The antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies.

Form 

Liquid

Concentration 

Batch dependent within range: 0.75 - 0.90 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for ADCY3 antibody (ab14778)

  This product has been used in:

Gonzalez-Iglesias AE et al. Dependence of electrical activity and calcium influx-controlled prolactin release on adenylyl cyclase signaling pathway in pituitary lactotrophs. Mol Endocrinol 20:2231-46 (2006). PubMed: 16645040
If you publish research using ab14778 please let us know so that we can cite the reference on this datasheet.

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