Recombinant
RabMAb

Anti-ADFP antibody [EPR3713] (ab108323)

Overview

  • Product name
    Anti-ADFP antibody [EPR3713]
    See all ADFP primary antibodies
  • Description
    Rabbit monoclonal [EPR3713] to ADFP
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-Fr or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human ADFP.

  • Positive control
    • Fetal liver, HepG2, JAR, Human milk, Mouse brain and Rat brain lysates; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab108323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-Fr or IP.
  • Target

    Images

    • Overlay histogram showing Jurkat cells stained with ab108323 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108323, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : JAR lysate
      Lane 4 : Human milk lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 48 kDa
    • ab108323 at 1/100 dilution staining ADFP in HepG2 cells by Immunofluorescence.
    • All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 48 kDa

    References

    This product has been referenced in:
    • Feaver RE  et al. Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis. JCI Insight 1:e90954 (2016). Read more (PubMed: 27942596) »
    • Xu Z  et al. Hepatitis B virus X induces inflammation and cancer in mice liver through dysregulation of cytoskeletal remodeling and lipid metabolism. Oncotarget 7:70559-70574 (2016). Read more (PubMed: 27708241) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (breast)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Specification
    breast
    Blocking step
    Ventana antibody dilution buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 35°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 14 2015

    Thank you for your call today and for letting us know about the trouble with ab108323.

    Please keep me updated about any new results or progress after trying the alterations that we discussed. We do fully guarantee this antibody to work in you...

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    Thank you for your inquiry.


    The immunogenpeptide for ab108323maps between amino acids 1 - 70 of human ADFP (UniProt: Q99541). We cannot guarantee that this antibody will work in an ELISA as this is untested, but theoretically if you fin...

    Read More

    Thank you for your patience.

    I have contacted the lab and, unfortunately, they confirmed that this antibody was tested for IHC and IP, but IHC staining was non-specific and IP results were negative.

    I hope this information is none...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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