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RabMAbs (3293-1)

Anti-ADFP antibody [EPR3713] (ab108323)

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Overview

  • Product nameAnti-ADFP antibody [EPR3713]See all ADFP primary antibodies ...
  • Description
    Rabbit monoclonal [EPR3713] to ADFP
  • Tested applicationsWB, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human ADFP.

  • Positive controlFetal liver, HepG2, JAR, Human milk, Mouse brain and Rat brain lysates; HepG2 cells.
  • General notesProduced under U.S. Patent No. 5,675,063.

Applications

Our Abpromise guarantee covers the use of ab108323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
Flow Cyt Flow Cyt: 1/100 - 1/500.
ICC/IF ICC/IF: 1/100 - 1/250. Antigen retrieval is recommended.
  • Application notesIs unsuitable for ,IHC-Fr or IP.

    • Target

    • Alternative names
        ADFP antibodyAdipophilin antibodyAdipose differentiation related protein antibody
        Adipose differentiation-related protein antibodyADRP antibodyMGC10598 antibodyPerilipin-2 antibodyPLIN2 antibodyPLIN2_HUMAN antibody
      see all

    Anti-ADFP antibody [EPR3713] images

    • Flow Cytometry - Anti-ADFP antibody [EPR3713] (ab108323)
      Flow Cytometry - Anti-ADFP antibody [EPR3713] (ab108323)
      Overlay histogram showing Jurkat cells stained with ab108323 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108323, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Western blot - ADFP antibody [EPR3713] (ab108323)
      Western blot - ADFP antibody [EPR3713] (ab108323)
      All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : JAR lysate
      Lane 4 : Human milk lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 48 kDa
    • Immunocytochemistry/ Immunofluorescence - ADFP antibody [EPR3713] (ab108323)
      Immunocytochemistry/ Immunofluorescence - ADFP antibody [EPR3713] (ab108323)
      ab108323 at 1/100 dilution staining ADFP in HepG2 cells by Immunofluorescence.
    • Western blot - ADFP antibody [EPR3713] (ab108323)
      Western blot - ADFP antibody [EPR3713] (ab108323)
      All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 48 kDa

    References for Anti-ADFP antibody [EPR3713] (ab108323)

    ab108323 has not yet been referenced specifically in any publications.

    Publishing research using ab108323 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab108323 Submit a Question for ab108323

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    High background in Western blot.

    Thank you for your call today and for letting us know about the trouble with ab108323.

    Please keep me updated about any new results or progress after trying the alterations that we discussed. We do fully guarantee this antibody to work in your a...

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    Hello,

    I have a question about the monoclonal Ab ab108323. I am trying to multiplex a few antibodies using a sandwich ELISA for a project and one of them is adipophilin. I would like to try this monoclonal as the capture antibody and I was wond...

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    Thank you for your inquiry.


    The immunogenpeptide for ab108323maps between amino acids 1 - 70 of human ADFP (UniProt: Q99541). We cannot guarantee that this antibody will work in an ELISA as this is untested, but theoretically if you find a...

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    We purchased the ADFP antibody (EPR3713) in August, 2011. The Product information sheet shows that it cannot be used for IHC. Can you please let us know if it has been upgraded to be used for IHC. Or, do you have any suggestion that confirms that it can b...

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    Thank you for your patience.

    I have contacted the lab and, unfortunately, they confirmed that this antibody was tested for IHC and IP, but IHC staining was non-specific and IP results were negative.

    I hope this information is nonethe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"