Anti-ADFP antibody [EPR3713] (ab108323)



Our Abpromise guarantee covers the use of ab108323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-Fr or IP.
  • Target

    Anti-ADFP antibody [EPR3713] images

    • Overlay histogram showing Jurkat cells stained with ab108323 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108323, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : JAR lysate
      Lane 4 : Human milk lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 48 kDa
    • ab108323 at 1/100 dilution staining ADFP in HepG2 cells by Immunofluorescence.
    • All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 48 kDa

    References for Anti-ADFP antibody [EPR3713] (ab108323)

    ab108323 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (breast)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
    Specification breast
    Blocking step Ventana antibody dilution buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 35°C
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted May 14 2015

    Thank you for your call today and for letting us know about the trouble with ab108323.

    Please keep me updated about any new results or progress after trying the alterations that we discussed. We do fully guarantee this antibody to work in you...

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    Thank you for your inquiry.

    The immunogenpeptide for ab108323maps between amino acids 1 - 70 of human ADFP (UniProt: Q99541). We cannot guarantee that this antibody will work in an ELISA as this is untested, but theoretically if you fin...

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    Thank you for your patience.

    I have contacted the lab and, unfortunately, they confirmed that this antibody was tested for IHC and IP, but IHC staining was non-specific and IP results were negative.

    I hope this information is none...

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