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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab37516? Please let us know so that we can cite the reference in this datasheet
ab37516 has been referenced in 3 publications.
- MacPherson RE et al. Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP at rest and following contraction in rat soleus muscle. Am J Physiol Regul Integr Comp Physiol 302:R29-36 (2012). PubMed: 22012700
- Sapiro JM et al. Hepatic triacylglycerol hydrolysis regulates PPAR-alpha activity. J Lipid Res : (2009). WB; Rat. PubMed: 19304987
- Jhaveri R et al. Domain 3 of hepatitis C virus core protein is sufficient for intracellular lipid accumulation. J Infect Dis 200:1781-8 (2009). ICC/IF; Rat. PubMed: 19852667
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - ADFP antibody (ab37516)
Anti-ADFP antibody (ab37516) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size : 48 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
A fusion protein was used in this western blot; hence the difference in molecular weight compared to native protein.
Immunocytochemistry/ Immunofluorescence - ADFP antibody (ab37516)
ab37516 staining ADFP in Human THP-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.05% saponin and blocked with 1% BSA for 2 hours at 22ºC. Samples were incubated with primary antibody (1/400 in 1% BSA, PBST) for 16 hours at 4ºC. An Alexa Fluor®568-conjugated goat anti-chicken monoclonal (1/500) was used as the secondary antibody. ADFP was localized on the periphery of small lipid droplets around the cell.
This image is courtesy of an anonymous Abreview
ADFP antibody for Western blot in Human (37516)
ADFP antibody for ICC/IF in Human (37516)
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