Overview

  • Product nameAnti-ADFP antibodySee all ADFP primary antibodies ...
  • Description
    Chicken polyclonal to ADFP
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide, corresponding to Internal sequence amino acids 71-240 of Human ADFP.

Properties

Applications

Our Abpromise guarantee covers the use of ab37516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: 1/400.
    WB: 1/2000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-ADFP antibody images

    • Anti-ADFP antibody (ab37516) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg

      Secondary
      Goat anti-IgY-HRP at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab37516 staining ADFP in Human THP-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.05% saponin and blocked with 1% BSA for 2 hours at 22°C. Samples were incubated with primary antibody (1/400 in 1% BSA, PBST) for 16 hours at 4°C. An Alexa Fluor®568-conjugated goat anti-chicken monoclonal (1/500) was used as the secondary antibody. ADFP was localized on the periphery of small lipid droplets around the cell.

      See Abreview

    References for Anti-ADFP antibody (ab37516)

    This product has been referenced in:
    • Vogt DA  et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB ; Human . Read more (PubMed: 23593007) »
    • MacPherson RE  et al. Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP at rest and following contraction in rat soleus muscle. Am J Physiol Regul Integr Comp Physiol 302:R29-36 (2012). Read more (PubMed: 22012700) »

    See all 5 Publications for this product

    Product Wall



    I am very pleased to hear you would like to accept our offer and testab37516 insheep. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forsheep and include this code in ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (THP-1)
    Loading amount 30 µg
    Specification THP-1
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 14 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (THP-1)
    Specification THP-1
    Fixative Formaldehyde
    Permeabilization Yes - 0.05% saponin
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 08 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Human fetal membranes (amniochorion) and decidua)
    Loading amount 40 µg
    Specification Human fetal membranes (amniochorion) and decidua
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 09 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"