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Products:Cardiovascular >> Hypoxia >> Hypoxia-Regulated
Overview
Product name
Anti-ADFP antibody
See all ADFP products (6) ...
Description
Chicken polyclonal to ADFP
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Synthetic peptide, corresponding to Internal sequence amino acids 71-240 of Human ADFP.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipid transport
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Cardiovascular >> Hypoxia >> Hypoxia-Regulated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/400.
WB: 1/2000. Predicted molecular weight: 48 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in development and maintenance of adipose tissue.
Tissue specificity
Milk lipid globules.
Sequence similarities
Belongs to the perilipin family.
Post-translational
modifications
Acylated; primarily with C14, C16 and C18 fatty acids.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ99541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
Database links
- Entrez Gene: 280981 Cow
- Entrez Gene: 123 Human
- Entrez Gene: 11520 Mouse
- Entrez Gene: 298199 Rat
- Omim: 103195 Human
- SwissProt: Q9TUM6 Cow
- SwissProt: Q99541 Human
- SwissProt: P43883 Mouse
see all
Anti-ADFP antibody images:
Western blot - ADFP antibody (ab37516)
Anti-ADFP antibody (ab37516) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size : 48 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
A fusion protein was used in this western blot; hence the difference in molecular weight compared to native protein.
Immunocytochemistry/ Immunofluorescence - ADFP antibody (ab37516)
ab37516 staining ADFP in Human THP-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.05% saponin and blocked with 1% BSA for 2 hours at 22°C. Samples were incubated with primary antibody (1/400 in 1% BSA, PBST) for 16 hours at 4°C. An Alexa Fluor®568-conjugated goat anti-chicken monoclonal (1/500) was used as the secondary antibody. ADFP was localized on the periphery of small lipid droplets around the cell.
This image is courtesy of an anonymous Abreview
References for Anti-ADFP antibody (ab37516)
This product has been referenced in:
- Sapiro JMet al. Hepatic triacylglycerol hydrolysis regulates PPAR-alpha activity. J Lipid Res : (2009). WB; Rat.Read more (PubMed: 19304987) »
- Jhaveri Ret al. Domain 3 of hepatitis C virus core protein is sufficient for intracellular lipid accumulation. J Infect Dis 200:1781-8 (2009). ICC/IF; Rat.Read more (PubMed: 19852667) »
See all 2 publications for this product
Publishing research using ab37516? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"