Overview
- Product nameAnti-ADFP antibodySee all ADFP primary antibodies ...
- DescriptionChicken polyclonal to ADFP
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Mouse, Rat, Cow, Human
- Immunogen
Synthetic peptide, corresponding to Internal sequence amino acids 71-240 of Human ADFP.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgY
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab37516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | |
| WB |
WB: 1/2000. Predicted molecular weight: 48 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionMay be involved in development and maintenance of adipose tissue.
- Tissue specificityMilk lipid globules.
- Sequence similaritiesBelongs to the perilipin family.
- Post-translational
modificationsAcylated; primarily with C14, C16 and C18 fatty acids. - Cellular localizationMembrane.
-
Database links
- Entrez Gene: 280981 Cow
- Entrez Gene: 123 Human
- Entrez Gene: 11520 Mouse
- Entrez Gene: 298199 Rat
- Omim: 103195 Human
- SwissProt: Q9TUM6 Cow
- SwissProt: Q99541 Human
- SwissProt: P43883 Mouse
- Unigene: 2568 Cow
- Unigene: 3416 Human
- Unigene: 381 Mouse
see all
Target information above from: UniProt accession
Q99541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- ADFP antibodyAdipophilin antibodyAdipose differentiation related protein antibody
- Adipose differentiation-related protein antibodyADRP antibodyMGC10598 antibodyPerilipin-2 antibodyPLIN2 antibodyPLIN2_HUMAN antibody
see all
Anti-ADFP antibody images
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Western blot - ADFP antibody (ab37516)Anti-ADFP antibody (ab37516) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size : 48 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - ADFP antibody (ab37516)This image is courtesy of an anonymous Abreviewab37516 staining ADFP in Human THP-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.05% saponin and blocked with 1% BSA for 2 hours at 22°C. Samples were incubated with primary antibody (1/400 in 1% BSA, PBST) for 16 hours at 4°C. An Alexa Fluor®568-conjugated goat anti-chicken monoclonal (1/500) was used as the secondary antibody. ADFP was localized on the periphery of small lipid droplets around the cell.
References for Anti-ADFP antibody (ab37516)
This product has been referenced in:
- MacPherson RE et al. Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP at rest and following contraction in rat soleus muscle. Am J Physiol Regul Integr Comp Physiol 302:R29-36 (2012). Read more (PubMed: 22012700) »
- Qiang G & Jhaveri R Lipid droplet binding of hepatitis C virus core protein genotype 3. ISRN Gastroenterol 2012:176728 (2012). ICC/IF ; Human . Read more (PubMed: 22844606) »
