Overview

  • Product nameAnti-ADFP antibodySee all ADFP primary antibodies ...
  • Description
    Chicken polyclonal to ADFP
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide, corresponding to Internal sequence amino acids 71-240 of Human ADFP.

Properties

Applications

Our Abpromise guarantee covers the use of ab37516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: 1/400.
    WB: 1/2000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-ADFP antibody images

    • Anti-ADFP antibody (ab37516) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg

      Secondary
      Goat anti-IgY-HRP at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab37516 staining ADFP in Human THP-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.05% saponin and blocked with 1% BSA for 2 hours at 22°C. Samples were incubated with primary antibody (1/400 in 1% BSA, PBST) for 16 hours at 4°C. An Alexa Fluor®568-conjugated goat anti-chicken monoclonal (1/500) was used as the secondary antibody. ADFP was localized on the periphery of small lipid droplets around the cell.

      See Abreview

    References for Anti-ADFP antibody (ab37516)

    This product has been referenced in:
    • Vogt DA  et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB ; Human . Read more (PubMed: 23593007) »
    • MacPherson RE  et al. Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP at rest and following contraction in rat soleus muscle. Am J Physiol Regul Integr Comp Physiol 302:R29-36 (2012). Read more (PubMed: 22012700) »

    See all 5 Publications for this product

    Product Wall



    I am very pleased to hear you would like to accept our offer and testab37516 insheep. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forsheep and include this code in ...

    Read More
    Application Western blot
    Sample Human Cell lysate - other (THP-1)
    Loading amount 30 µg
    Specification THP-1
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Submitted Jun 14 2010

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (THP-1)
    Specification THP-1
    Fixative Formaldehyde
    Permeabilization Yes - 0.05% saponin
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

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    Verified customer

    Submitted Apr 08 2010

    Application Western blot
    Sample Human Tissue lysate - whole (Human fetal membranes (amniochorion) and decidua)
    Loading amount 40 µg
    Specification Human fetal membranes (amniochorion) and decidua
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Jul 09 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"