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Products:Cardiovascular >> Hypoxia >> Hypoxia-Regulated
Overview
Product name
Anti-ADFP antibody
See all ADFP products (6) ...
Description
Rabbit polyclonal to ADFP
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Pig, Monkey
Immunogen
A synthetic peptide made to an internal portion of the human ADFP protein, within residues 150-250.
Positive control
Human liver lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 30% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipid transport
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Cardiovascular >> Hypoxia >> Hypoxia-Regulated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
ICC/IF: Use at an assay dependent dilution (PMID 19920176).
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in development and maintenance of adipose tissue.
Tissue specificity
Milk lipid globules.
Sequence similarities
Belongs to the perilipin family.
Post-translational
modifications
Acylated; primarily with C14, C16 and C18 fatty acids.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ99541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
Database links
- Entrez Gene: 280981 Cow
- Entrez Gene: 123 Human
- Entrez Gene: 11520 Mouse
- Entrez Gene: 397402 Pig
- Entrez Gene: 298199 Rat
- Omim: 103195 Human
- SwissProt: Q9TUM6 Cow
- SwissProt: Q99541 Human
see all
Anti-ADFP antibody images:
Western blot - ADFP antibody (ab52355)
Anti-ADFP antibody (ab52355) at 2 µg/ml + Human liver lysate
Predicted band size : 48 kDa
Observed band size : 48 kDa
Immunocytochemistry/ Immunofluorescence - ADFP antibody (ab52355)
ab52355 staining ADFP in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50: in PBS; 1% BSA) for 1 hour at 25°C. An Alexa Fluor®568-conjugated Goat polyclonal to rabbit IgG, dilution 1/100, was used as secondary antibody.
This image is courtesy of an Abreview submitted by Dr. Francesca Lolicato
References for Anti-ADFP antibody (ab52355)
This product has been referenced in:
- Aardema Het al. Oleic acid prevents detrimental effects of saturated fatty acids on bovine oocyte developmental competence. Biol Reprod 85:62-9 (2011). WB, ICC/IF; Human.Read more (PubMed: 21311036) »
- Hinson ER & Cresswell P The antiviral protein, viperin, localizes to lipid droplets via its N-terminal amphipathic alpha-helix. Proc Natl Acad Sci U S A 106:20452-7 (2009). WB, ICC/IF; Human.Read more (PubMed: 19920176) »
See all 2 publications for this product
Publishing research using ab52355? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"