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Western blot - ADFP antibody (ab52355)
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Immunocytochemistry/ Immunofluorescence - ADFP antibody (ab52355)
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Product Name
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ADFP antibody
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See all ADFP antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADFP
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Immunogen
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A synthetic peptide made to an internal portion of the human ADFP protein, within residues 150-250.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Cow, Mk, Pig
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 19920176).
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver lysate
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Cellular localization
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Peripheral membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipid transport
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Cardiovascular >> Hypoxia >> Hypoxia-Regulated
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Relevance
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Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP) associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, was a major constituent of the globule surface and was present in a detergent-insoluble complex that contained stoichiometric amounts of butyrophilin and xanthine oxidase.
ADFP (Adipophilin) occurs in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to certain cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 30% Glycerol, PBS Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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0.980 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADFP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADFP antibody images, references, products related to ab52355 and other tools.
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ADFP antibody images:
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 Western blot - ADFP antibody (ab52355)
ADFP antibody (ab52355) at 2 µg/ml + Human liver lysate
Predicted band size : 48 kDa
Observed band size : 48 kDa
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Immunocytochemistry/ Immunofluorescence - ADFP antibody (ab52355)
ab52355 staining ADFP in HeLa cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50: in PBS; 1% BSA) for 1 hour at 25°C. An Alexa Fluor®568-conjugated Goat polyclonal to rabbit IgG, dilution 1/100, was used as secondary antibody. This image is courtesy of an Abreview submitted by Dr. Francesca Lolicato
See Abreview
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References for ADFP antibody (ab52355)
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This product has been used in:
Hinson ER & Cresswell P The antiviral protein, viperin, localizes to lipid droplets via its N-terminal amphipathic alpha-helix. Proc Natl Acad Sci U S A 106:20452-7 (2009). WB, ICC/IF; Human. PubMed: 19920176
If you publish research using ab52355 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ADFP
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ADFP antibody - more information
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Customer reviews (feedback) regarding ADFP antibody |
Customer FAQs regarding ADFP antibody |
Protocols for ADFP antibody |
Price and availability of products related to ADFP antibody |
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ADFP antibody - related products:
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ADFP antibody - other tools:
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Contact Abcam with a Technical Enquiry about ADFP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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