Feedback Form

Western blot - ADFP antibody (ab52356)
Western blot - ADFP antibody (ab52356)
(enlarge)


Product Name 

ADFP antibody

 

See all ADFP antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADFP

Immunogen 

A synthetic peptide made to a C-terminal region within residues 350-425 of the mouse ADFP protein.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• WB

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)


Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse and human liver lysate

Cellular localization 

Peripheral membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipid transport
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Cardiovascular >> Hypoxia >> Hypoxia-Regulated

Relevance 

Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP) associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, was a major constituent of the globule surface and was present in a detergent-insoluble complex that contained stoichiometric amounts of butyrophilin and xanthine oxidase.
ADFP (Adipophilin) occurs in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to certain cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

 

Alternative names ADFP antibody (ab52356)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

123    (Human)
11520    (Mouse)
298199    (Rat)

GeneCard

    

ADFP    (Human)

Omim

    

103195    (Human)

SwissProt

    

Q99541    (Human)
Q5SYF3    (Human)
Q5SYF4    (Human)
Q6FHZ7    (Human)
Q541S0    (Mouse)
P43883    (Mouse)
Q5U2U5    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 30% Glycerol, PBS

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

0.840 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADFP antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ADFP antibody images, references, products related to ab52356 and other tools.

 

ADFP antibody images:

Product image  Western blot - ADFP antibody (ab52356)

Western blot - ADFP antibody (ab52356)

ADFP antibody (ab52356) at 2 µg/ml + mouse liver lysate

Predicted band size : 48 kDa
Observed band size : 48 kDa

 
 

Search PubMed (MEDLINE) for references to ADFP

 

ADFP antibody - more information

Customer reviews (feedback) regarding ADFP antibody

Customer FAQs regarding ADFP antibody

Protocols for ADFP antibody

Price and availability of products related to ADFP antibody

 

ADFP antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for ADFP antibody

ab29299

  

Liver (Mouse) Membrane Lysate - normal tissue

ab29879

  

Liver (Human) Membrane Lysate - adult normal tissue

 

Compatible Secondaries for ADFP antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other ADFP Antibodies

See all ADFP antibodies (4)...

 

ADFP antibody - other tools:

Contact Abcam with a Technical Enquiry about ADFP antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.