Overview
- Product nameAnti-ADFP antibodySee all ADFP primary antibodies ...
- DescriptionRabbit polyclonal to ADFP
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Pig, Orangutan - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ADFP.
(Peptide available as ab90212.)
- Positive controlAb78920 gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293. This antibody also gave a positive signal in Mouse Heart Tissue Lysates.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab78920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with ADFP peptide (ab90212). |
Target
- FunctionMay be involved in development and maintenance of adipose tissue.
- Tissue specificityMilk lipid globules.
- Sequence similaritiesBelongs to the perilipin family.
- Post-translational
modificationsAcylated; primarily with C14, C16 and C18 fatty acids. - Cellular localizationMembrane.
-
Database links
- Entrez Gene: 123 Human
- Entrez Gene: 11520 Mouse
- Entrez Gene: 397402 Pig
- Omim: 103195 Human
- SwissProt: Q99541 Human
- SwissProt: P43883 Mouse
- SwissProt: Q4PLW0 Pig
- Unigene: 3416 Human
- Unigene: 381 Mouse
- Unigene: 6323 Pig
see all
Target information above from: UniProt accession
Q99541
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- ADFP antibodyAdipophilin antibodyAdipose differentiation related protein antibody
- Adipose differentiation-related protein antibodyADRP antibodyMGC10598 antibodyPerilipin-2 antibodyPLIN2 antibodyPLIN2_HUMAN antibody
see all
Anti-ADFP antibody images
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Western blot - ADFP antibody (ab78920)All lanes : Anti-ADFP antibody (ab78920) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 48 kDa
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes -
Immunocytochemistry/ Immunofluorescence - ADFP antibody (ab78920)ICC/IF image of ab78920 stained HepG2 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78920, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa, and Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, and HepG2 cells at 1µg/ml -
Western blot - Anti-ADFP antibody (ab78920)Anti-ADFP antibody (ab78920) at 1 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 48 kDa
Exposure time : 2 minutes -
Western blot - ADFP protein (T7 Tag) (ab73631)Anti-ADFP antibody (ab78920) at 1 µg/ml +ADFP protein (T7 Tag) (ab73631) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
References for Anti-ADFP antibody (ab78920)
ab78920 has not yet been referenced specifically in any publications.
