Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Metabolism >> Alcohol metabolism
Overview
Product name
Anti-ADH1B antibody
See all ADH1B products (4) ...
Description
Rabbit polyclonal to ADH1B
Specificity
This antibody reacts with ADH1B
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee
Immunogen
A region within synthetic peptide: TSVIVGVPPA SQNLSINPML LLTGRTWKGA VYGGFKSKEG IPKLVADFMA, corresponding to C terminal amino acids 289-338 of Human ADH1B
TSVIVGVPPA SQNLSINPML LLTGRTWKGA VYGGFKSKEG IPKLVADFMA
Positive control
Jurkat cell lysate and human kidney tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1:1562500
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00325
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADH beta subunit antibody
- ADH1B antibody
- ADH1B_HUMAN antibody
see all
Database links
- Entrez Gene: 125 Human
- Omim: 103720 Human
- SwissProt: P00325 Human
- Unigene: 4 Human
Anti-ADH1B antibody images:
Western blot - ADH1B antibody (ab58647)
Anti-ADH1B antibody (ab58647) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 40 kDa
Observed band size : 40 kDa
Immunohistochemistry (Paraffin-embedded sections) - ADH1B antibody (ab58647)
ab58647 at a 4.0µg/ml dilution staining ADH1B in the epithelial cells of human renal tubules. Arrows indicate positively labeled epithelial cells of renal tubules.
References for Anti-ADH1B antibody (ab58647)
ab58647 has not yet been referenced specifically in any publications.
Publishing research using ab58647? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"