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Western blot - ADH1B antibody (ab58647)
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Immunohistochemistry (Paraffin-embedded sections) - ADH1B antibody (ab58647)
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Product Name
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ADH1B antibody
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See all ADH1B antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADH1B
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Immunogen
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A region within synthetic peptide: TSVIVGVPPA SQNLSINPML LLTGRTWKGA VYGGFKSKEG IPKLVADFMA, corresponding to C terminal amino acids 289-338 of Human ADH1B
BLAST 'TSVIVGVPPA SQNLSINPML LLTGRTWKGA VYGGFKSKEG IPKLVADFMA ' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with ADH1B
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1:1562500
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate and human kidney tissue
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Alcohol metabolism
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Relevance
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ADH1B is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.
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Database links
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The links below go to external sites and will open in a new browser window | SwissProt | | P00325 (Human)
| Unigene | | 4 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADH1B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADH1B antibody images, references, products related to ab58647 and other tools.
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ADH1B antibody images:
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 Western blot - ADH1B antibody (ab58647)
ADH1B antibody (ab58647) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 40 kDa
Observed band size : 40 kDa
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Immunohistochemistry (Paraffin-embedded sections) - ADH1B antibody (ab58647)
ab58647 at a 4.0µg/ml dilution staining ADH1B in the epithelial cells of human renal tubules. Arrows indicate positively labeled epithelial cells of renal tubules.
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Search PubMed (MEDLINE) for references to ADH1B
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ADH1B antibody - more information
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Customer reviews (feedback) regarding ADH1B antibody |
Customer FAQs regarding ADH1B antibody |
Protocols for ADH1B antibody |
Price and availability of products related to ADH1B antibody |
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ADH1B antibody - related products:
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ADH1B antibody - other tools:
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Contact Abcam with a Technical Enquiry about ADH1B antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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