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Products:Signal Transduction >> Metabolism >> Alcohol metabolism
Overview
Product name
Anti-ADH1C antibody
Description
Mouse polyclonal to ADH1C
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length Human ADH1C protein (NP_000660.1, 1 a.a. ~ 375 a.a).
Positive control
ADH1C transfected 293T cell lysate; human liver tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Alcohol metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Target
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00326
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alcohol dehydrogenase 3 antibody
- Class I alcohol dehydrogenase antibody
- ADH gamma subunit antibody
see all
Database links
- Entrez Gene: 126 Human
- Omim: 103730 Human
- SwissProt: P00326 Human
- Unigene: 654537 Human
Anti-ADH1C antibody images:
Western blot - ADH1C antibody (ab76745)
Anti-ADH1C antibody (ab76745) at 1 µg/ml + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 40 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot - ADH1C antibody (ab76745)
All lanes : Anti-ADH1C antibody (ab76745) at 1 µg/ml
Lane 1 : ADH1C transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 40 kDa
Observed band size : 40 kDa
References for Anti-ADH1C antibody (ab76745)
ab76745 has not yet been referenced specifically in any publications.
Publishing research using ab76745? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"