Recombinant Anti-ADH5 antibody [EPR12885(B)] (ab177932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12885(B)] to ADH5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ADH5 antibody [EPR12885(B)]
See all ADH5 primary antibodies -
Description
Rabbit monoclonal [EPR12885(B)] to ADH5 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ADH5 aa 1-100. The exact sequence is proprietary.
Database link: P11766 -
Positive control
- WB: Human liver, Mouse liver, Human fetal liver, HepG2, Human fetal kidney, SH-SY5Y, Mouse brain, Rat liver, and Rat brain lysates. IHC-P: human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12885(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/50-1/100 |
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa. |
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/50-1/100 |
Target
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Function
Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 128 Human
- Entrez Gene: 11532 Mouse
- Entrez Gene: 100145871 Rat
- Omim: 103710 Human
- SwissProt: P11766 Human
- SwissProt: P28474 Mouse
- SwissProt: P12711 Rat
- Unigene: 78989 Human
see all -
Alternative names
- ADH 3 antibody
- ADH5 antibody
- ADHX antibody
see all
Images
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Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified) + Human liver lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling ADH5 with purified ab177932 at 1/8000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified)
Lane 1 : Mouse liver lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat liver lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaADH5 is highly expressed in liver, kidney and low expressed in brain as is described in PMID: 26412304
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All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/1000 dilution ((unpurified))
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : Human fetal kidney lysates
Lane 4 : SH-SY5Y lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab177932 has been referenced in 3 publications.
- Chen G et al. bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway. Redox Biol 41:101904 (2021). PubMed: 33706169
- Pan L et al. S-Nitrosylation of Plastin-3 Exacerbates Thoracic Aortic Dissection Formation via Endothelial Barrier Dysfunction. Arterioscler Thromb Vasc Biol 40:175-188 (2020). PubMed: 31694393
- Chen W et al. Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. J Cell Mol Med 23:8161-8172 (2019). PubMed: 31565850