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Products:Signal Transduction >> Metabolism >> Alcohol metabolism
Overview
Product name
Anti-ADHFE1 antibody
See all ADHFE1 products (3) ...
Description
Mouse polyclonal to ADHFE1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length human ADHFE1, (NP_653251, 419 amino acids).
Positive control
ADHFE1 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Alcohol metabolism
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Western blot - ADHFE1 antibody (ab70868)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight for isoform 2: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ADHFE1 belongs to the iron-containing alcohol dehydrogenase family, Hydroxyacid-oxoacid transhydrogenase subfamily. ADHFE1 catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for ADHFE1 with 10-fold lower activities. There are four named isoforms.
Cellular localization
Mitochondrial
Alternative names
- Fe containing alcohol dehydrogenase antibody
- ADH8 antibody
- ADHFE 1 antibody
see all
Database links
- Entrez Gene: 137872 Human
- Omim: 611083 Human
- SwissProt: Q8IWW8 Human
Anti-ADHFE1 antibody images:
Western blot - ADHFE1 antibody (ab70868)
All lanes : Anti-ADHFE1 antibody (ab70868) at 1/500 dilution
Lane 1 : ADHFE1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 45 kDa
Observed band size : 45 kDa
References for Anti-ADHFE1 antibody (ab70868)
ab70868 has not yet been referenced specifically in any publications.
Publishing research using ab70868? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"