Overview

  • Product name
  • Description
    Rabbit polyclonal to ADI1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-179 of Human ADI1 (UniProt: Q9BV57).

  • Positive control
    • HepG2, Mouse lung and Rat lung lysates; HeLa cells; Human CL1-5 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Has aci-reductone dioxygenase (ARD) activity and can function in the 5-methylthioadenosine (MTA) methionine salvage pathway. Down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line.
  • Tissue specificity
    Detected in heart, colon, lung, stomach, brain, spleen, liver, skeletal muscle and kidney.
  • Pathway
    Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.
  • Sequence similarities
    Belongs to the acireductone dioxygenase (ARD) family.
  • Cellular localization
    Cell membrane. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 2 dihydroxy 3 keto 5 methylthiopentene dioxygenase antibody
    • 1 antibody
    • 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase antibody
    • Aci reductone dioxygenase antibody
    • Acireductone dioxygenase (Fe(2+) requiring) antibody
    • Acireductone dioxygenase (Ni(2+) requiring) antibody
    • Acireductone dioxygenase (Ni(2+)-requiring) antibody
    • Acireductone dioxygenase 1 antibody
    • ADI 1 antibody
    • Adi1 antibody
    • ADI1 antibody antibody
    • APL1 antibody
    • ARD antibody
    • EC 1.13. antibody
    • Fe ARD antibody
    • FLJ10913 antibody
    • HMFT1638 antibody
    • Membrane type 1 matrix metalloproteinase cytoplasmic tail binding protein 1 antibody
    • Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1 antibody
    • MT1 MMP cytoplasmic tail binding protein 1 antibody
    • MTCBP 1 antibody
    • MTCBP-1 antibody
    • MTCBP1 antibody
    • MTND_HUMAN antibody
    • Ni-ARD antibody
    • SIPL antibody
    • Submergence induced protein 2 antibody
    • Submergence induced protein 2 homolog antibody
    • Submergence induced protein like factor antibody
    • Submergence-induced protein 2 homolog antibody
    see all

Images

  • Anti-ADI1 antibody (ab154689) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 21 kDa



    12 % SDS-PAGE
  • Anti-ADI1 antibody (ab154689) at 1/1000 dilution + Mouse lung lysate at 50 µg

    Predicted band size: 21 kDa



    12 % SDS-PAGE
  • Anti-ADI1 antibody (ab154689) at 1/1000 dilution + Rat lung lysate at 50 µg

    Predicted band size: 21 kDa



    12 % SDS-PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling ADI1 with ab154689 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human CL1-5 xenograft tissue labeling ADI1 with ab154689 at 1/500 dilution.

References

ab154689 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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