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Abcam's ADP Assay Kit (Colorimetric/Fluorometric) provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (λmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable.
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Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories.
|ADP Assay Buffer||WM||1 x 25ml|
|ADP Converter (lyophilized)||Purple||1 vial|
|ADP Developer Mix (lyophilized)||Green||1 vial|
|ADP Probe (in DMSO)||Red||1 x 200µl|
|ADP Standard (1 µmole) (lyophilized)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab83359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ADP levels measured fluorometrically in cell culture supernatants (background signal subtracted, mean of duplicates; +/- SD).
ADP levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
APD levels colorimetrically measured in biological fluids (rat plasma and serum, human saliva); background signal subtracted, mean of duplicates; +/- SD.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"