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Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)
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Product Name
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ADP Colorimetric/Fluorometric Assay Kit
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Product type
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Kits
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Description
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ADP Colorimetric/Fluorometric Assay Kit
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Product overview
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Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. This newly designed ADP Assay Kit provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (Lambda max = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1 µM ADP in biological samples.
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Components
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| Kit Components | Unit Size/Volume | Cap Colour |
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| ADP Assay Buffer | 25ml | WM | | ADP Probe (lyophilized) | 1 vial | Red | | Dimethylsulfoxide (DMSO, Anhydrous) | 0.4 ml | Brown | | ADP Converter | 1 vial | Purple | | ADP Developer Mix | 1 vial | Green | | ATP Standard (1 µmol; lyophilized) | 1 vial | Yellow |
Note: Please Click Here for Instructions For Use/Protocol
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Tested applications
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FuncS
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Notes
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Protect kit from light. Briefly centrifuge all vials prior to opening. Warm ADP Assay Buffer to room temperature prior to use.
ADP Probe: Store reconstituted probe at –20°C, protect from light and moisture. Use within two months.
ADP Converter and ADP Developer Mix: Store reconstituted components at –20°C. Use within two months.
ADP Standard: Store reconstituted ADP standard at -20°C. Keep cold while in use.
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Material safety datasheet (MSDS) for this product:
ADP Assay Kit MSDS
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Relevance
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ADP (Adenosine diphosphate) is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADP Colorimetric/Fluorometric Assay Kit is on this datasheet. But please do contact us if you would like any reassurance! |
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ADP Colorimetric/Fluorometric Assay Kit images:
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 Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)
Colorimetric and Fluorometric examples of ADP Standard curve.
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Search PubMed (MEDLINE) for references to ADP
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ADP Colorimetric/Fluorometric Assay Kit - more information
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Customer reviews (feedback) regarding ADP Colorimetric/Fluorometric Assay Kit |
Customer FAQs regarding ADP Colorimetric/Fluorometric Assay Kit |
Protocols for ADP Colorimetric/Fluorometric Assay Kit |
Price and availability of products related to ADP Colorimetric/Fluorometric Assay Kit |
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ADP Colorimetric/Fluorometric Assay Kit - other tools:
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Contact Abcam with a Technical Enquiry about ADP Colorimetric/Fluorometric Assay Kit |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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