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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
ADP Colorimetric/Fluorometric Assay Kit
Product overview
Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. This newly designed ADP Assay Kit provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (Lambda max = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1 µM ADP in biological samples.
Notes
Protect kit from light. Briefly centrifuge all vials prior to opening. ADP Assay Buffer should be stored at –20°C and warmed to room temperature prior to use.
ADP Probe: Store reconstituted probe at –20°C, protect from light and moisture. Use within two months.
ADP Converter and ADP Developer Mix: Store reconstituted components at –20°C. Use within two months.
ADP Standard: Store reconstituted ADP standard at -20°C. Keep cold while in use.
ADP Probe: Store reconstituted probe at –20°C, protect from light and moisture. Use within two months.
ADP Converter and ADP Developer Mix: Store reconstituted components at –20°C. Use within two months.
ADP Standard: Store reconstituted ADP standard at -20°C. Keep cold while in use.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
| Components | Identifier | 100 tests |
|---|---|---|
| ADP Assay Buffer | WM | 1 x 25ml |
| ADP Converter (lyophilized) | Purple | 1 vial |
| ADP Developer Mix (lyophilized) | Green | 1 vial |
| ADP Probe (in DMSO) | Red | 1 x 200µl |
| ADP Standard (1 µmole) (lyophilized) | Yellow | 1 vial |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Intermediary Metabolism Kits
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Signal Transduction >> Metabolism >> Energy Metabolism
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Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)
(enlarge)
Relevance
ADP (Adenosine diphosphate) is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
Alternative names
- Adenosine diphosphate
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
ADP Colorimetric/Fluorometric Assay Kit images:
Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)
Colorimetric and Fluorometric examples of ADP Standard curve.
References for ADP Colorimetric/Fluorometric Assay Kit (ab83359)
ab83359 has not yet been referenced specifically in any publications.
Publishing research using ab83359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"