ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)
- Product nameADP Assay Kit (Colorimetric/Fluorometric)
- Tests100 x 1 assay
- Sample typeUrine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
- Assay typeQuantitative
- Sensitivity> 1 µM
- Assay time1h 00m
- Product overview
Abcam's ADP Assay Kit (Colorimetric/Fluorometric) provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (λmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable.
Visit our FAQs page for tips and troubleshooting.
Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests ADP Assay Buffer WM 1 x 25ml ADP Converter (lyophilized) Purple 1 vial ADP Developer Mix (lyophilized) Green 1 vial ADP Probe (in DMSO) Red 1 x 200µl ADP Standard (1 µmole) (lyophilized) Yellow 1 vial
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
- Adenosine diphosphate
Our Abpromise guarantee covers the use of ab83359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ADP Assay Kit (Colorimetric/Fluorometric) images
Colorimetric and Fluorometric examples of ADP Standard curve.
References for ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)
This product has been referenced in:
- Mayeur S et al. Maternal calorie restriction modulates placental mitochondrial biogenesis and bioenergetic efficiency: putative involvement in fetoplacental growth defects in rats. Am J Physiol Endocrinol Metab 304:E14-22 (2013). Functional Studies . Read more (PubMed: 23092912) »