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ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)

Q&A (9)Specific References (1)

Overview

  • Product nameADP Assay Kit (Colorimetric/Fluorometric)
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 1 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's ADP Assay Kit (Colorimetric/Fluorometric) provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (λmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceADP (Adenosine diphosphate) is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
    • Alternative names
        Adenosine diphosphate

    Applications

    Our Abpromise guarantee covers the use of ab83359 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    ADP Assay Kit (Colorimetric/Fluorometric) images

    • Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)
      Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)
      Colorimetric and Fluorometric examples of ADP Standard curve.

    Protocols

    References for ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)

    This product has been referenced in:
    • Mayeur S  et al. Maternal calorie restriction modulates placental mitochondrial biogenesis and bioenergetic efficiency: putative involvement in fetoplacental growth defects in rats. Am J Physiol Endocrinol Metab 304:E14-22 (2013). Functional Studies . Read more (PubMed: 23092912) »

    See 1 Publication for this product

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    Displaying 1 - 9 of 9 results for Abreviews and Q&A

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    Does 2.5% perchloric acid infer with the ADP assay kit (ab83359)? I would like to pre-treat my samples with 2.5% perchloric acid to remove protein prior to testing for ADP.

    Thank you for your inquiry.

    I would recommend the spin column filters (ab93349) over the PCA treatment for deproteinization. Theoretically the PCA treatment should be fine, but since we have not tested the outcome of this assay with such treated...

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    I want to use this kit to study the conversion to ADP, so using Poly A and an enzyme and then detecting ADP rather than testing in cells or tissue. Do you think this will work?

    Inquiry: I am looking for a rapid assay to detect CDP or IDP. Would...

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    Thank you for contacting us.

    Although the laboratory has not tested in the conditions you describe, using a reaction with an enzyme, they believe their is no compatibility issue and that it should work with some optimization. Same goes for the ...

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    deproteinization - necessary for these kits?

    Merci pour votre appel de vendredi.

    Le laboratoire m'a pu confirmer que vous pouvez procéder sans lescolonnes:

    Pour le kit ADP ab83359, les échantillons lysés dans le"ADP assay buffer" peuventêtre a...

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    what is assay buffer composition?

    sample: purified protein in buffer (Tris, NaCl, TCEP (protease inhibitor) and 200 uM ATP)
    the buffer by itself gives high background (OD570 = 0.5 or higher for 100 ul buffer)
    What could be the problem...

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    Unfortunately we do not give out our buffer components or compositions. Sorry about that.

    Yes, it is very likely that the ATP in your buffer is giving the background. I would suggest doing a background control for each of the experimental sample...

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    what is assay buffer composition?

    sample: purified protein in buffer (Tris, NaCl, TCEP (protease inhibitor) and 200 uM ATP)
    the buffer by itself gives high background (OD570 = 0.5 or higher for 100 ul buffer)
    What could be the problem...

    Read More

    Thank you for contacting us.

    I apologize for the wait, but I have not yet heard back from the lab. I have sent another email to follow up with them.

    Thank you for your patience and understanding.

    are sample lysates in Tris, EDTA compatible with kit?
    can dilute samples in assay buffer or need to prepare samples again just in assay buffer?

    Thank you for contacting us.

    I have checked with the lab and their advice is as follows:

    For most efficient results, we always recommend that the samples be made in the assay buffer provided with the kit. In case of that not being po...

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    Does the assay buffer in both kits same?

    Thank you for contacting us.

    I can confirm that the assay buffers in both the kits are different. These cannot be used interchangeably. If you are running out of buffers then let us know we can provide them separately.

    I hope this in...

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    Thank you for this information. We have bought all three kits. I have some follow up questions.
    Are the buffers from these three kits are exchangeable too?
    Can the ADP converter enzyme from ADP/ATP ratio kits (ab 65313) be replaced by the ATP co...

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    Thank you for your questions. My colleague is out of the office today but I would like to forward the response that we received from the lab:

    Unfortunately, these three kits have different Assay buffers and converting enzyme mixes, so they can n...

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    Has this kit been tested with bacteria or yeast?

    Thank you for your inquiry. This kit has not been tested with bacterial cells by the lab, however theoretically as long as the cells are prepared as indicated in the attached data sheet, it should work with such samples.  I hope this information hel...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"