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Anti-ADP Ribosylation Factor antibody [1D9] (ab2806)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab2806 for help.

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2 questions for ab2806

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Question 1

Thursday 07-December-2006

If I am using a human whole cell lysate, how much of each type of Arf is detected? Is there a paper showing the relative intensities of each Arf band in a Western?

ANSWER:

  

Thank you for your enquiry. Unfortunately we have not done testing to quantify the relative amounts of ARF isotypes from human whole cell lysate at this time. I apologize for any inconvenience this may cause you.

I do have one article that specifically references this antibody in WB which may be of use. You may find it at the link below.

http://jvi.asm.org/cgi/reprint/79/11/7207

I do wish that I had more information. I hope that this information helps and please don't hesitate to contact us for more advice or information.

Question 2

Wednesday 23-March-2005

ab2806 supposes to detect 21kDa by Western Blot, but positive results using heart extract showing about 10kDa in your website. I want to know that reason. Also, I want to know whether this Ab can detect the single band of endogeneous expression. If so, please tell me the example. f.e, Hella cell 30ug. Finally, I want to ask whether this Ab will detect the single band even it detected Arf1,3,4,5 and 6.

ANSWER:

  

Thank you for your enquiry. After reviewing the data, the molecular weight markers on this data sheet appear to be incorrect. The band should be between the 14 KDa and 20 KDa, as indicated by Swiss Prot. There are many different forms of ARF and all forms can be modified on the N-Terminus. Depending on the species used and the form of ARF present, the molecular weights of the protein can vary from the mid teens, to the mid twenties. The image has been removed from this data sheet, whilst the originator of this product amends the information. If you have any further questions then please get back in contact with me.

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