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Read our guarantee »Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Anti-ADP Ribosylation Factor antibody [1D9]
Mouse monoclonal [1D9] to ADP Ribosylation Factor
Detects ADP-ribosylation factor1 (Arf 1), Arf 3, Arf 5 and Arf 6 and at about a ten-fold reduction with Arf 4.
ICC/IF, WB, Flow Cyt, IPmore details
Reacts with
Mouse, Rat, Hamster, Dog, Human, Fruit fly (Drosophila melanogaster)
Recombinant human Arf 1
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: PBS
Concentration information loading...
Ascites
Monoclonal
1D9
IgG1
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Our Abpromise guarantee covers the use of ab2806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500
WB: Use at an assay dependent dilution.
Flow Cyt: 1/20
IP: Use at an assay dependent dilution.
The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.
Golgi Apparatus
Immunoprecipitation - ADP Ribosylation Factor antibody [1D9] (ab2806)
![Immunoprecipitation - ADP Ribosylation Factor antibody [1D9] (ab2806)](/ps/datasheet/Images/2/ab2806/ab2806_2.jpg)
Ab2806 immunoprecipitating ADP Ribosylation Factor from HeLa cells. 10ul of antibody were added to 500ul of HeLa cell lysate (at 1.5mg/ml) and incubated overnight before precipitation with Protein G. Negative control lysate received the same treatment except no antibody was added. ab2806 was used at 1/500 in the western blotting stage and the secondary antibody was an HRP conjugated anti-mouse IgG. The membrane was finally stained with amido black.
Lane 1: negative control
Lane 2: ab2806
This image is courtesy of an Abreview submitted by Denis Krndija submitted on 5 January 2006.
Western blot - ADP Ribosylation Factor antibody [1D9] (ab2806)
![Western blot - ADP Ribosylation Factor antibody [1D9] (ab2806)](/ps/datasheet/images/2/ab2806/ADP-Ribosylation-Factor-Primary-antibodies-ab2806-4.jpg)
All lanes : Anti-ADP Ribosylation Factor antibody [1D9] (ab2806) at 1/500 dilution
Lane 1 : Lysates prepared from human Huh-7 cells
Lane 2 : Lysates prepared from human Huh-7 cells
Lysates/proteins at 15000 cells per lane.
Secondary
HRP-conjugated sheep polyclonal to mouse IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Exposure time : 4 minutes
This image is a courtesy of Anonymous Abreview
Flow Cytometry-ADP Ribosylation Factor antibody [1D9](ab2806)
](/ps/datasheet/images/2/ab2806/ADP-Ribosylation-Factor-Primary-antibodies-ab2806-5.jpg)
Overlay histogram showing HeLA cells stained with ab2806 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2806, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
See all 8 publications for this product
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![Immunoprecipitation - ADP Ribosylation Factor antibody [1D9] (ab2806)](/ps/datasheet/Images/2/ab2806/ab2806_2.jpg)
Ab2806 immunoprecipitating ADP Ribosylation Factor from HeLa cells. 10ul of antibody were added to 500ul of HeLa cell lysate (at 1.5mg/ml) and incubated overnight before precipitation with Protein G. Negative control lysate received the same treatment except no antibody was added. ab2806 was used at 1/500 in the western blotting stage and the secondary antibody was an HRP conjugated anti-mouse IgG. The membrane was finally stained with amido black.
Lane 1: negative control
Lane 2: ab2806
This image is courtesy of an Abreview submitted by Denis Krndija submitted on 5 January 2006.
![Western blot - ADP Ribosylation Factor antibody [1D9] (ab2806)](/ps/datasheet/images/2/ab2806/ADP-Ribosylation-Factor-Primary-antibodies-ab2806-4.jpg)
All lanes : Anti-ADP Ribosylation Factor antibody [1D9] (ab2806) at 1/500 dilution
Lane 1 : Lysates prepared from human Huh-7 cells
Lane 2 : Lysates prepared from human Huh-7 cells
Lysates/proteins at 15000 cells per lane.
Secondary
HRP-conjugated sheep polyclonal to mouse IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Exposure time : 4 minutes
This image is a courtesy of Anonymous Abreview
](/ps/datasheet/images/2/ab2806/ADP-Ribosylation-Factor-Primary-antibodies-ab2806-5.jpg)
Overlay histogram showing HeLA cells stained with ab2806 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2806, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
3
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