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Immunoprecipitation - ADP Ribosylation Factor antibody [1D9] (ab2806)
![Immunoprecipitation - ADP Ribosylation Factor antibody [1D9] (ab2806)](/ps/datasheet/images/ab2806_2.jpg)
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Western blot - ADP Ribosylation Factor antibody [1D9] (ab2806)
![Western blot - ADP Ribosylation Factor antibody [1D9] (ab2806)](/ps/datasheet/images/2/ab2806/ADP-Ribosylation-Factor-Primary-antibodies-ab2806-4.jpg)
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Product Name
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ADP Ribosylation Factor antibody [1D9]
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [1D9] to ADP Ribosylation Factor
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Immunogen
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Recombinant human Arf 1
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Reacts with
(species key)
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Hu, Ms, Rat, Dog, Hm
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Specificity
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Detects ADP-ribosylation factor1 (Arf 1), Arf 3, Arf 5 and Arf 6 and at about a ten-fold reduction with Arf 4.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Immunofluorescence: 1/500.
Western Blot: 1/500.
Immunoprecipitation: 1/50 (see Abreview).
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract.
Immunofluorescence staining of Arf in rat pancreas results in staining of the cytoplasmic face of the trans-Golgi membranes.
This antibody cannot be used to neutralize Arf in solution.
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Cellular localization
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Golgi Apparatus
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Research areas
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Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Relevance
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The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.
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ADP Ribosylation Factor antibody [1D9] (ab2806) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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1D9
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADP Ribosylation Factor antibody [1D9] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADP Ribosylation Factor antibody [1D9] images, references, products related to ab2806 and other tools.
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ADP Ribosylation Factor antibody [1D9] images:
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 Immunoprecipitation - ADP Ribosylation Factor antibody [1D9] (ab2806)
Ab2806 immunoprecipitating ADP Ribosylation Factor from HeLa cells. 10ul of antibody were added to 500ul of HeLa cell lysate (at 1.5mg/ml) and incubated overnight before precipitation with Protein G. Negative control lysate received the same treatment except no antibody was added. ab2806 was used at 1/500 in the western blotting stage and the secondary antibody was an HRP conjugated anti-mouse IgG. The membrane was finally stained with amido black. Lane 1: negative control Lane 2: ab2806 This image is courtesy of an Abreview submitted by Denis Krndija submitted on 5 January 2006.
See Abreview
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Western blot - ADP Ribosylation Factor antibody [1D9] (ab2806)
All lanes : ADP Ribosylation Factor antibody [1D9] (ab2806) at 1/500 dilution
Lane 1 : Lysates prepared from human Huh-7 cells
Lane 2 : Lysates prepared from human Huh-7 cells
Lysates/proteins at 15000 cells per lane.
Secondary
HRP-conjugated sheep polyclonal to mouse IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Exposure time : 4 minutes This image is a courtesy of Anonimous Abreview
See Abreview
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References for ADP Ribosylation Factor antibody [1D9] (ab2806)
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This product has been used in: (two most recent references)
Kametaka S et al. Functional characterization of protein-sorting machineries at the trans-Golgi network in Drosophila melanogaster. J Cell Sci 123:460-71 (2010). PubMed: 20067992
Anthonio EA et al. Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6. BMC Cell Biol 10:58 (2009). PubMed: 19686593
See all 5 publication references for this product.
If you publish research using ab2806 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ADP Ribosylation Factor
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ADP Ribosylation Factor antibody [1D9] - more information
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ADP Ribosylation Factor antibody [1D9] - related products:
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ADP Ribosylation Factor antibody [1D9] - other tools:
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Contact Abcam with a Technical Enquiry about ADP Ribosylation Factor antibody [1D9] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunoprecipitation - ADP Ribosylation Factor antibody [1D9] (ab2806)](/index.html?datasheet=2806#ab2806_2.jpg)
![Western blot - ADP Ribosylation Factor antibody [1D9] (ab2806)](/index.html?datasheet=2806#ADP-Ribosylation-Factor-Primary-antibodies-ab2806-4.jpg)
